11. Trouble Shooting

 

 

Problem

Ursache/Abhilfe

 

 

Hellfeld

 

 

 

Das Präparat ist nicht zu fokussieren.

￿Verwenden Sie das korrekte Immersions-

 

medium.

 

￿Legen Sie das Präparat mit dem Deckglas

 

nach oben.

 

￿Stellen Sie sicher, dass die Deckglasdicke

 

korrekt ist und mit den Angaben am Objektiv

 

übereinstimmt.

Dunkelfeld

 

 

 

Es lässt sich kein eindeutiger DF-Kontrast ein- stellen.

￿Stellen Sie sicher, dass ein DF-Objektiv ver- wendet wird.

￿Die Objektiv-Apertur ist zu hoch (maximal 0,75). Objektiv-Apertur eventuell durch Iris- blende am Objektiv reduzieren.

￿Überprüfen Sie die Kondensorzentrierung.

Das Bild ist inhomogen/ungleichmäßig ausge- leuchtet.

￿Die Objektivvergrößerung ist zu schwach. Wählen Sie eine höhere Vergrößerung.

Unerwünschte Lichtstreuung.

￿Säubern Sie das Präparat und die angrenzen-

 

den Linsenflächen (S. 77).

Phasenkontrast

Es lässt sich kein Phasenkontrast einstellen.

￿Das Präparat ist zu dick.

￿Das Deckglas ist nicht gleichmäßig aufgelegt. ￿Überprüfen Sie die Zentrierung der Lichtringe

(S. 44).

75

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Image 155
Leica DM4500P, DM5000B, DM4000M manual Nach oben, Übereinstimmt, Dunkelfeld, Phasenkontrast

DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

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