11. Troubleshooting

Problem

Cause/remedy

 

 

Bright field

The specimen can not be brought into focus.

Dark field

￿Use the correct immersion medium.

￿Lay the specimen with the cover glass toward the top.

￿Make sure that the cover glass thickness is correct and that it meets the specifications on the objective.

No definite DF contrast is possible.

￿Be sure that a DF objective is being used.

￿The objective aperture setting is too high (maximum 0.75). If necessary, reduce the objective aperture using the iris diaphragm on the objective.

￿Check the condenser centering.

The image is unevenly or not uniformly

￿The magnification is too weak.

illuminated.

Use a higher magnification.

 

 

Undesirable stray light.

￿Clean the specimen and neighboring lens sur-

 

faces (p. 77).

Phase contrast

 

 

 

No phase contrast is possible.

￿The specimen is too thick.

 

￿The cover glass is not placed planar.

 

￿Check the centering of the light rings

 

(p. 44).

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Leica DM5000B manual Troubleshooting Problem Cause/remedy Bright field, Specimen can not be brought into focus, Dark field

DM4500P, DM5000B, DM4000M specifications

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