11. Troubleshooting

Problem

Cause/remedy

 

 

Polarization

No polarization contrast is possible.

￿Bring the polarizer and analyzer into cross po- sition until they reach maximum darkness (without specimen). (p. 61ff, 72).

￿The embedding material for transmitted light specimen is birefringent.

The image is insufficient.

￿Polarizers can be damaged by strong light

 

sources. Check to see if the polarizer is dam-

 

aged (discolored) or heavily soiled.

The image is too dark.

￿Check to see if a beam splitter or filter is switched on.

No conoscopy image is possible.

￿Objectives and condensers can be deformed by mechanical damage.

Fluorescence

The image is completely dark (no fluorescence). ￿Open the shutter (p. 70).

￿Select the incident light axis (IL) (p. 40). ￿Check the antigen-antibody combination.

The fluorescence is too weak.

Display

￿Insert the booster (p. 33). ￿Center the lamp (p. 47ff). ￿Insert a new lamp (p. 23f).

The display flashes.

￿Iotate an appropriate objective for the con-

 

trast method into place.

 

 

FAIL! appears on the display.

￿Check the installed objectives, filter cubes, etc. ￿Switch the microscope off and back on.

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Leica DM4000M, DM5000B, DM4500P manual Troubleshooting Problem Cause/remedy Polarization, Display

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