7. Startup

7.6 Checking the phase contrast rings

If your microscope is equipped for the use of phase contrast, the light rings that fit the objec- tives are built into the condenser.

The light rings are already centered in the facto- ry. However, the centering should be rechecked.

•In the place of an eyepiece, insert the focus- ing telescope (Fig. 43) into the observation tube.

•Rotate the phase contrast objective with the lowest magnification into place.

•Focus on the specimen with the focus wheel .

Note:

Every objective is assigned its own light ring in the condenser disk. Therefore, a check must be performed for each objective. When rotating in an objective that is suitable for phase contrast, the corresponding light ring is set automatically.

•Press the BF (bright field) button (one of the variable function keys, to the left behind the focus wheels).

•Focus the ring structure (44a.a) by slightly loosening the clamping ring (43.2) and moving the eyelens (43.1).

•Retighten the clamping ring.

•Press the PH (Phase Contrast) button. The ring diaphragm (light ring) in the condenser is turned inward.

•If the light ring and the phase ring are not shown as arranged in Fig. 44a.c, the light ring must be centered.

 

 

Fig. 44a Phase contrast centering procedure

 

 

PH=phase contrast ring, LR=light ring

 

 

a Condenser in bright field (BF) position

Fig. 43 Focusing telescope

b Condenser in phase contrast (PH) position

1

Adjustable eyelens

Light ring (LR) not centered

2

Clamping ring for fixing the focus position

c Light ring and phase ring centered

a

b

c

1

 

 

2

 

 

44

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Page 44
Image 44
Leica DM4500P, DM5000B, DM4000M manual Startup Checking the phase contrast rings, Focus on the specimen with the focus wheel
Page 43 Page 45

DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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