7. Startup

•Using the condenser height adjuster (39.4), adjust the condenser until the edge of the field diaphragm appears in sharp relief.

•If the image does not appear in the middle of the field of view (41c), the condenser must be moved into the middle of the field of view with the help of the two centering screws (40.1).

•Open the field diaphragm just enough for it to disappear from the field of view (41d).

Caution:

The condenser height adjustment depends on the thickness of the specimen. It may need to be adjusted for each specimen.

Fig. 41 Köhler illumination

aField diaphragm not focused, not centered

bField diaphragm focused, but not centered

cField diaphragm focused and centered Diameter is too small, however

dField diameter (light) = Field diameter (view) (Köhler illumination)

ab

7.5.2 Incident light

Suitable aperture and field diaphragm values have been preset for each objective. The inci- dent light module has also been centered at the factory.

However, it may be necessary to readjust the incident light module in some cases after trans- porting and setting up the stand. Therefore, check the aperture and field diaphragm center- ing.

The following procedure is provided for the inci- dent light-bright field illumination.

•Select an objective with moderate magnifica- tion (10x-20x).

•Activate the incident-light axis with the TL/IL button (38.1).

"IL" appears in the first line of the display.

•Select the bright field contrast method by pressing the IL-BFbutton (DM4000 M) or se- lect fluorescence by pressing the FLUO button (DM4000 B, DM5000 B).

These functions can be assigned to the vari- able function keys on the stand.

IL BF / FLUO appears in the first line of the dis- play.

•Insert the specimen in the stage’s specimen holder (39.3).

•Focus on the specimen with the focus wheels.

•Adjust the light intensity using the INT buttons (38.3).

cd

42

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Image 42
Leica DM5000B, DM4000M, DM4500P manual Incident light, Adjust the light intensity using the INT buttons
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DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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