8. Operation

Rotating the stage

The swivel range of the rotating stages is 0° - 110°.

•Rotate the stage to loosen the locking screw (59b.1).

•Bring the table into the desired position.

•Retighten the locking screw.

Pol rotating stage (360°)*, Pol attachable me- chanical stage *

The specimen can be fastened using either two spring-back stage clips or the Pol 3 multi-format attachable mechanical stage (Fig. 59a). For glass slides of approx. 26 mm (1") in width, the metal plate (59a.2) needs to be pivoted outwards and the specimen needs to be positioned ac- cording to Fig. 59a. If commercially available glass slides of 26 mm in width are placed per- pendicular to that, then the range of movement of the attachable mechanical stage, which is normally approx. 30 mm x 40 mm, is not fully used. The pair of lock buttons provided allow for locking distances of 0.1, 0.3, 0.5, 1 and 2 mm. Changing them requires forceful, axial removal. When attaching the new lock button, make sure that the catch pins on the inside are properly positioned. For smaller microscopes, the limit stop screw on the underside must be shifted approx. 2 mm inwards toward the stroke limit.

The two verniers allow the angular measure- ments to be read with a precision of 0.1.

45°click stop:

•Screw in the rotary knob (59a.5) until you can feel it starting to resist.

•Then turn the specimen stage to the next click stop.

•Loosen the rotary knob and look for the start- ing point of the next click stop (e.g., the object darkness setting).

•Retighten the rotary knob.

Now the rotating stage can be turned using 45° click stop intervals.

Fig. 59a Pol rotating stage* and Pol 3 attachable mechani- cal stage*

1Holes for the fastening screw.

2Lever for the holder for glass slides of various formats, which can be turned inward and outward

3Storage for the centering key

4Locking button pair

545° click stop

6Clamping system for the stage rotation

4

3

1

2

5

6

52

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Leica DM4000M, DM5000B, DM4500P Operation Rotating the stage, Pol rotating stage 360*, Pol attachable me- chanical stage
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DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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