8. Operation

•Switch off the analyzer, the 1.6x tube lens and the Bertrand lens.

•Reduce the aperture diaphragm so that it is very small.

•Insert both objective centering keys above the objectives that need to be centered.

•Focus the specimen.

Two resembling methods can be used for cen- tering objectives:

Method I (Fig. 64)

•Turn the specimen stage and note the position of the specimen that does not move in a circu- lar path. This position of the specimen corre- sponds to the mechanical axis of rotation of the specimen stage.

•Now move the marked specimen position by shifting the two centering keys to the center of the cross-hairs.

•Turn the specimen stage and refine the cen- tering as needed.

Fig. 64 Centering method I Fig. 65 Centering method II

Method II (Fig. 65)

•Move the marked specimen position (65a) into the center of the M cross-hairs.

•Turn the specimen stage until the specimen position is as far from the center of the M cross-hairs as possible (Position A, Fig. 65b). In extreme cases, point A (= maximum devia- tion of the specimen position) can also be lo- cated outside of the field of view.

•Move the image by turning the centering key until position A of the specimen is located in the center (= Pos. B) between pos. A and the center of the M cross-hairs (65c).

•Move position A of the specimen to M and check to see whether A remains in M when the stage is rotated (65d). If necessary, repeat the centering procedure.

The objective centering procedure needs to be repeated for each objective. This ensures that the objectives retain their approximate center- ing settings when they are removed for clean- ing, or other such procedures, and then rein- serted into the same holes. If the coarse drive or the height adjustment device is used to change the height of the specimen stage (for example, when viewing thick specimens) the centering precision for all of the objectives may be reduced slightly.

M

 

M

M

M

A

 

B

 

B

 

A

 

A

a

b

c

 

d

57

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Image 57
Leica DM5000B, DM4000M, DM4500P manual Method II Fig, Centering method I Centering method
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DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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