9. Contrast methods for Leica DM4000 B/DM4500 P/DM5000 B

•For fine adjustment, use the knurled wheel above the objective turret.

9.2 Fluorescence

•Switch to the fluorescence axis / fluores- cence cube (FLUO) by pushing the TL/IL func- tion key.

•Apply a specimen and rotate a suitable objec- tive into place.

•The current fluorescence filter cube is indi- cated on the display.

•You may protect your specimen from fading by closing the incident light shutter.

Do so by pressing the SHUTTER variable but- ton.

(For details on key assignments, please see

the identification sheet.)

↓

 

This symbol appears on the display:

•Changing the fluorescence filter cube: By pressing the variable key

Cube or Cube

•Bring the image into focus using the focus wheel and set the brightness using the INT function key.

•The fluorescence intensity can be increased using the booster lens on the right side of the stand (Fig. 75). Leica recommends that you in- sert the booster lens into the front slot.

•For multifluorescence, use of an excitation manager is recommended. The excitation manager is inserted into the stand up to the last click stop on the right side of the stand (Fig. 76). Leica recommends that you insert the excitation manager into the front slot.

Fig. 75 Inserting the booster lens

Fig. 76 Inserting the excitation manager

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Leica DM4000M, DM5000B, DM4500P manual Fluorescence, Do so by pressing the Shutter variable but- ton, Cube or Cube
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DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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