Contents

Contents

1.

Important notes about this manual

7

2.

Intended purpose of the microscope ....

8

3.

Safety notes

9

3.1

General safety notes

9

3.2

Electrical safety

10

3.3

Disposal

11

4.

Overview of the instrument

12

5.

Unpacking the microscope

17

6.

Assembling the microscope

19

6.1

Specimen stage

19

6.2

Condenser

21

6.3

Tube and eyepieces

22

6.4

Objectives

23

6.5

Light sources for the transmitted

 

 

light axis

23

6.6

Light sources for the incident

 

 

light axis

25

6.7

Equipping the incident light

 

 

turret disk

30

6.8

Polarizer and analyzer

31

6.9

DIC prisms

32

6.10

Optional accessories

33

6.11

Connection to the power supply

34

6.12

Connecting to the CTR5000

 

 

electronics box

34

7.

Startup

35

7.1

Functional principle

35

7.2

Switching on the unit

38

7.3

The display

 

 

(DM4000 B/4500 B/4000 M/4500 P)

39

7.4

The function keys

40

7.5

Köhler illumination

41

 

7.5.1

Transmitted light

41

 

7.5.2

Incident light

42

7.6. Checking the phase contrast rings

44

7.7

Setting the motorized polarizer

 

 

(DM4500 P/DM5000 B)

45

7.8

Adjusting the light sources

45

8.

Operation

51

8.1

Switching on the unit

51

8.2

Stages and object displacement

51

8.3

Focusing

53

8.4

Tubes

53

8.5

Eyepieces

55

8.6

Objectives

55

8.7

Magnification changer

58

8.8

HC P 1x/1.6x tube optics

58

8.9

Light sources

59

8.10

Aperture diaphragm and

 

 

field diaphragm

59

5

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Leica DM4500P, DM5000B, DM4000M manual Contents

DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.