6
Contents
9. Contrast methods for
Leica DM4000 B/DM4500 B/
DM4500 P/DM5000 B ................................ 60
9.1 Transmitted light........................................ 60
9.1.1 Bright field......................................... 60
9.1.2 Phase contrast.................................. 60
9.1.3 Dark field............................................ 61
9.1.4 Polarization........................................ 61
9.1.4.1 Manual method ............................. 61
9.1.4.2 DM4500 P - examinations
in polarized transmitted light...... 62
9.1.4.3 Motorized method ........................ 68
9.1.4.4 Combined methods....................... 68
9.1.5 Differential interference
contrast ............................................ 68
9.1.5.1 DM4500 B/DM4500 P.................... 68
9.1.5.2 DM5000 B........................................ 69
9.2 Fluorescence.............................................. 70
10. Contrast methods for
Leica DM4000 M........................................ 71
10.1 Incident light.............................................. 71
10.1.1 Bright field....................................... 71
10.1.2 Dark field.......................................... 71
10.1.3 Polarization...................................... 72
10.1.4 Interference contrast.................... 73
10.2 Transmitted light........................................ 73
10.2.1 Bright field....................................... 73
10.2.2 Polarization...................................... 73
11. Troubleshooting......................................... 74
12. Care of the microscope ........................... 77
12.1 Dust cover................................................... 77
12.2 Cleaning....................................................... 77
12.3 Handling acids and bases....................... 78
13. Essential wear and spare parts............. 79
14. Abbreviations and pictograms............... 80
15. Index ............................................................ 8 1
16. EU Declaration of Conformity................. 82