Contents

9.

Contrast methods for

 

11.

 

Troubleshooting

74

 

Leica DM4000 B/DM4500 B/

 

 

 

 

 

 

DM4500 P/DM5000 B

60

12.

 

Care of the microscope

77

9.1

Transmitted light

60

12.1

Dust cover

77

 

9.1.1 Bright field

60

12.2

Cleaning

77

 

9.1.2 Phase contrast

60

12.3

Handling acids and bases

78

 

9.1.3 Dark field

61

 

 

 

 

 

9.1.4 Polarization

61

13.

 

Essential wear and spare parts

79

 

9.1.4.1 Manual method

61

 

 

 

 

 

9.1.4.2 DM4500 P - examinations

 

14.

 

Abbreviations and pictograms

80

 

in polarized transmitted light

62

 

 

 

 

 

9.1.4.3 Motorized method

68

15.

 

Index

81

 

9.1.4.4 Combined methods

68

 

 

 

 

 

9.1.5 Differential interference

 

16.

EU Declaration of Conformity

82

 

contrast

68

 

 

 

 

 

9.1.5.1 DM4500 B/DM4500 P

68

 

 

 

 

 

9.1.5.2 DM5000 B

69

 

 

 

 

9.2

Fluorescence

70

 

 

 

 

10.

Contrast methods for

 

 

 

 

 

 

Leica DM4000 M

71

 

 

 

 

10.1

Incident light

71

 

 

 

 

 

10.1.1 Bright field

71

 

 

 

 

 

10.1.2 Dark field

71

 

 

 

 

 

10.1.3 Polarization

72

 

 

 

 

 

10.1.4 Interference contrast

73

 

 

 

 

10.2

Transmitted light

73

 

 

 

 

 

10.2.1 Bright field

73

 

 

 

 

 

10.2.2 Polarization

73

 

 

 

 

6

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Leica DM5000B Contents Contrast methods for, Leica DM4000 B/DM4500 B, 4.2 DM4500 P examinations, Differential interference

DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.