7. Startup

Insert the centering key through the corre- sponding openings (44b.1) on both sides of the condenser.

Turn the centering key until the dark ring (phase ring in the objective) is congruent with the slightly narrower bright ring (light ring in condenser) (44a.c).

Note:

When changing the objective, the centering key must no longer be in the corresponding open- ings.

Repeat the process for all other phase con- trast objectives.

Always remove the centering keys after the centering procedure.

7.7Setting the motorized polarizers (DM4500 P/DM5000 B)

Select the POL method (one of the variable function keys on the stand or on the Leica- Screen).

Insert the centering key into the correspond- ing openings (44b.2) on the condenser.

Set the optimum extinction (max. darkness).

7.8 Adjusting the light sources

Transmitted-light axis (TL) with lamp housing 107/2

The lamp housing 107/2 with a 12 V 100 W halo- gen lamp is fixed. Centering the lamp is not re- quired.

Fig. 44b Light ring centering

1Centering key in the phase contrast centering hole

2Polarization contrast centering hole

Fig. 45 Reflector cube for lamp adjustment

1

2

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Leica DM5000B, DM4000M, DM4500P manual Set the optimum extinction max. darkness, Reflector cube for lamp adjustment

DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

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