7. Startup

Adjusting the field diaphragm

Close the field diaphragm with the FD button (38.4) or manually until the edge of the dia- phragm (round or rectangular) appears in the field of view.

If the border of the field diaphragm does not appear in the middle of the field of view, the field diaphragm must be moved into the middle of the field of view using the two centering screws (42a.1) on the right side of the stand.

Use the function buttons FD (38.4) to open the field diaphragm to the point that they just dis- appear from the field of view.

We recommend the use of a rectangular field diaphragm when using a digital camera. Match the size of the diaphragm to the chip size of the camera.

Adjusting the aperture diaphragm (for DM4000 M and DM4500 P only)

Remove one eyepiece (e.g. right).

Close the aperture diaphragm with the AP function key (38.2) until the edge of the dia- phragm appears in the exit pupil of the objec- tive (aperture diaphragm plane).

If the image is not in the middle of the field of view of the exit pupil, move the position of the aperture diaphragm to the center of the exit pupil using the two centering screws (42b.2) located on the left side of the stand.

Open the aperture diaphragm to cover 2/3 of the field of view.

Fig. 42a Adjusting the field diaphragm in the incident light

Fig. 42b Adjusting the aperture diaphragm in the incident

axis

light axis

1 Adjusting screws for moving the field diaphragm

1 Adjusting screws for moving the aperture diaphragm

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Leica DM4000M, DM5000B, DM4500P manual Startup Adjusting the field diaphragm, Remove one eyepiece e.g. right

DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.