9. Contrast methods for Leica DM4000 B/DM4500 P/DM5000 B

9.Contrast methods for Leica DM4000 B/DM4500 P/DM5000 B

9.1Transmitted light 9.1.1 Bright field (TL)

Switch to the transmitted light axis (TL) by pushing the TL/IL function key.

Select the BF (bright field) contrast method. Do so by pressing the BF variable key. Alternatively: press the variable button

CHANGE TL .

(For details on key assignments, please see the identification sheet.)

The display indicates BF.

Insert a transmitted light specimen.

Rotate an appropriate objective into place.

Use the focus wheel to bring the image into focus and set the brightness using the INT function key.

9.1.2Phase contrast (TL)

Switch to the transmitted light axis (TL) by pushing the TL/IL function key.

Select the PH (phase contrast) contrast meth- od.

Do so by pressing the variable button PH. Alternatively: press the variable button

CHANGE TL .

(For details on key assignments, please see the identification sheet.)

The display indicates PH.

Insert a transmitted light specimen.

Rotate an appropriate objective into place. Objectives that are suitable for phase con- trast are engraved with PH.

Bring the image into focus using the focus wheel and set the brightness using the INT function key.

Notes:

The microscope automatically selects the cor- rect light ring in the condenser.

When selecting the phase contrast method, the aperture diaphragm is opened fully and can not be adjusted.

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Leica DM5000B, DM4000M, DM4500P manual Contrast methods for Leica DM4000 B/DM4500 P/DM5000 B, Phase contrast TL

DM4500P, DM5000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

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