Optimizing Performance

Optimize the Compressibility Compensation Setting

 

Optimize the Compressibility

 

Compensation Setting

 

The compressibility compensation default setting is 100 × 10-6 /bar for the

 

quaternary pump. This setting represents an average value. Under normal

 

conditions the default setting reduces the pressure pulsation to values

 

(below 1% of system pressure) that will be sufficient for most applications

 

and for all gradient analyses. For applications using sensitive detectors, the

 

compressibility settings can be optimized by using the values for the various

 

solvents described in Table 6. If the solvent in use is not listed in the

 

compressibility tables, when using isocratic mixtures of solvents and if the

 

default settings are not sufficient for your application the following

 

procedure can be used to optimize the compressibility settings.

 

 

N O T E

When using mixtures of solvents it is not possible to calculate the

 

compressibility of the mixture by interpolating the compressibility values of

 

the pure solvents used in that mixture or by applying any other calculation. In

 

these cases the following empirical procedure has to be applied to optimize

 

your compressibility setting.

 

 

1Start the quaternary pump with the required flow rate.

2Before starting the optimization procedure, the flow must be stable. Use degassed solvent only. Check the tightness of the system with the pressure test (see “Pressure Test” on page 82).

3Your pump must be connected to a Chemstation or a handheld controller, the pressure and %-ripple can be monitored with one of these instruments,

otherwhise connect a signal cable between the pressure output of the quaternary pump and a recording device (for example, 339X integrator) and set parameters.

Zero 50%

Att 2^3

Chart Speed 10 cm/min

4Start the recording device with the plot mode.

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Agilent Technologies 1100 Series manual Optimize the Compressibility, Compensation Setting
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1100 Series specifications

Agilent Technologies 1100 Series is a highly regarded lineup of high-performance liquid chromatography (HPLC) systems designed to meet the demanding needs of laboratories in various fields including pharmaceuticals, environmental testing, and food safety. Known for its versatility, reliability, and advanced technology, the 1100 Series has established itself as a benchmark for analytical performance.

One of the standout features of the Agilent 1100 Series is its modular architecture. This design allows users to configure the system according to specific analytical requirements. The components such as pumps, detectors, and autosamplers can be mixed and matched to create a tailored solution, ensuring maximum efficiency and optimal performance. This modularity also facilitates easy upgrades and maintenance, significantly extending the life cycle of the instrument.

At the heart of the 1100 Series is its advanced pumping technology. The system utilizes precise, high-pressure pumps that ensure a consistent flow rate and excellent reproducibility, essential for accurate quantification of analytes. The binary and quaternary pump options provide flexibility in method development, enabling users to perform gradient elution with ease.

The Agilent 1100 Series also boasts a range of detectors, including UV/VIS, fluorescence, and refractive index detectors. Each detector is designed to enhance sensitivity and specificity, enabling the detection of a wide array of compounds. The UV/VIS detector, in particular, is known for its reliable performance and user-friendly interface, which simplifies method setup and data analysis.

Another critical aspect of the 1100 Series is its autosampler. The system's autosamplers provide high throughput and automatic sample handling, which significantly reduces human error and increases productivity. The intelligent design allows for temperature control and reliable sample injection, ensuring optimal results.

In terms of software, the 1100 Series is supported by Agilent’s OpenLAB software, which offers an intuitive platform for method development, data acquisition, and analysis. This software integrates seamlessly with the HPLC hardware, making it easier for scientists to manage workflows and maintain compliance with regulatory requirements.

Overall, the Agilent Technologies 1100 Series represents a significant advancement in liquid chromatography, bringing together ideal characteristics such as modularity, precision, versatility, and sophistication. Its commitment to innovation and quality makes it a preferred choice for laboratories seeking reliable analytical solutions.
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