Troubleshooting and Test Functions

Evaluating the Results

First plateau negative, second plateau positive

Potential Cause

Corrective Action

 

 

Leaking outlet valve.

Clean the outlet valve. Ensure the sieve in the

 

outlet valves are installed correctly. Tighten the

 

outlet valve.

Loose pump head screws.

Ensure the pump head screws are tight.

Leaking seals or scratched plungers.

Exchange the pump seals. Check the plunger for

 

scratches. Exchange if scratched.

 

 

Ramp 3 does not reach limit

Potential Cause

Corrective Action

Pump stopped due to error.

Large leaks (visible) at the pump seals.

Large leaks (visible) at active inlet valve, outlet valve, or purge valve.

Check the logbook for error messages.

Exchange the pump seals.

Ensure the leaky components are installed tightly. Exchange the component if required.

Third plateau negative (pressure drop > 2 bar/min)

Potential Cause

Corrective Action

 

 

Loose or leaky fittings.

Ensure all fittings are tight, or exchange

 

capillary.

Loose purge valve.

Tighten the purge valve (14mm wrench).

Contaminated purge valve.

Open and close purge valve to flush out

 

contamination. Exchange the valve if still

 

leaky.

Loose pump head screws.

Ensure the pump head screws are tight.

Leaking seals or scratched plungers.

Exchange the pump seals. Check the plungers

 

for scratches. Exchange if scratched.

Leaking outlet valve.

Exchange the outlet valve.

Leaky damper.

Exchange damper.

 

 

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Agilent Technologies 1100 Series manual First plateau negative, second plateau positive, Ramp 3 does not reach limit

1100 Series specifications

Agilent Technologies 1100 Series is a highly regarded lineup of high-performance liquid chromatography (HPLC) systems designed to meet the demanding needs of laboratories in various fields including pharmaceuticals, environmental testing, and food safety. Known for its versatility, reliability, and advanced technology, the 1100 Series has established itself as a benchmark for analytical performance.

One of the standout features of the Agilent 1100 Series is its modular architecture. This design allows users to configure the system according to specific analytical requirements. The components such as pumps, detectors, and autosamplers can be mixed and matched to create a tailored solution, ensuring maximum efficiency and optimal performance. This modularity also facilitates easy upgrades and maintenance, significantly extending the life cycle of the instrument.

At the heart of the 1100 Series is its advanced pumping technology. The system utilizes precise, high-pressure pumps that ensure a consistent flow rate and excellent reproducibility, essential for accurate quantification of analytes. The binary and quaternary pump options provide flexibility in method development, enabling users to perform gradient elution with ease.

The Agilent 1100 Series also boasts a range of detectors, including UV/VIS, fluorescence, and refractive index detectors. Each detector is designed to enhance sensitivity and specificity, enabling the detection of a wide array of compounds. The UV/VIS detector, in particular, is known for its reliable performance and user-friendly interface, which simplifies method setup and data analysis.

Another critical aspect of the 1100 Series is its autosampler. The system's autosamplers provide high throughput and automatic sample handling, which significantly reduces human error and increases productivity. The intelligent design allows for temperature control and reliable sample injection, ensuring optimal results.

In terms of software, the 1100 Series is supported by Agilent’s OpenLAB software, which offers an intuitive platform for method development, data acquisition, and analysis. This software integrates seamlessly with the HPLC hardware, making it easier for scientists to manage workflows and maintain compliance with regulatory requirements.

Overall, the Agilent Technologies 1100 Series represents a significant advancement in liquid chromatography, bringing together ideal characteristics such as modularity, precision, versatility, and sophistication. Its commitment to innovation and quality makes it a preferred choice for laboratories seeking reliable analytical solutions.