Optimizing Performance

Hints for Successful Use of the Quaternary Pump

Hints for Successful Use of the Quaternary Pump

•Always place the solvent cabinet with the solvent bottles on top of the quaternary pump (or at a higher level).

•When using salt solutions and organic solvents in the Agilent 1100 Quaternary Pump it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports. It is best to have the organic channel directly above the salt solution channel. Regular flushing with water of all MCGV channels is recommended to remove all possible salt deposits in the valve ports.

•Before operating the quaternary pump flush the vacuum degasser with at least two volumes (30 ml), especially when turned off for a certain length of time (for example, during the night) and volatile solvent mixtures are used in the channels (see “Priming and Purging the System” on page 28).

•Prevent blocking of solvent inlet filters (never use the pump without solvent inlet filter). Growth of algae should be avoided (see “Prevent Blocking of Solvent Filters” on page 35).

•Check purge valve frit and column frit in regular time intervals. A blocked purge valve frit can be identified by black or yellow layers on its surface or by a pressure greater than 10 bar, when pumping distilled water at a rate of 5 ml/min with an open purge valve.

•When using the quaternary pump at low flow rates (for example,

0.2ml/min) check all 1/16-inch fittings for any signs of leaks.

•Whenever exchanging the pump seals the purge valve frit should be exchanged, too.

•When using buffer solutions, flush the system with water before switching it off. The seal wash option should be used when buffer concentrations of

0.1Molar or higher will be used for long time periods.

•Check the pump plungers for scratches when changing the plunger seals. Scratched plungers will lead to micro leaks and will decrease the lifetime of the seal.

•Pressurize the system according to the wear in procedure after changing the plunger seals (see “Exchanging the Pump Seals and Seal Wear-in

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Agilent Technologies 1100 Series manual Hints for Successful Use of the Quaternary Pump
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1100 Series specifications

Agilent Technologies 1100 Series is a highly regarded lineup of high-performance liquid chromatography (HPLC) systems designed to meet the demanding needs of laboratories in various fields including pharmaceuticals, environmental testing, and food safety. Known for its versatility, reliability, and advanced technology, the 1100 Series has established itself as a benchmark for analytical performance.

One of the standout features of the Agilent 1100 Series is its modular architecture. This design allows users to configure the system according to specific analytical requirements. The components such as pumps, detectors, and autosamplers can be mixed and matched to create a tailored solution, ensuring maximum efficiency and optimal performance. This modularity also facilitates easy upgrades and maintenance, significantly extending the life cycle of the instrument.

At the heart of the 1100 Series is its advanced pumping technology. The system utilizes precise, high-pressure pumps that ensure a consistent flow rate and excellent reproducibility, essential for accurate quantification of analytes. The binary and quaternary pump options provide flexibility in method development, enabling users to perform gradient elution with ease.

The Agilent 1100 Series also boasts a range of detectors, including UV/VIS, fluorescence, and refractive index detectors. Each detector is designed to enhance sensitivity and specificity, enabling the detection of a wide array of compounds. The UV/VIS detector, in particular, is known for its reliable performance and user-friendly interface, which simplifies method setup and data analysis.

Another critical aspect of the 1100 Series is its autosampler. The system's autosamplers provide high throughput and automatic sample handling, which significantly reduces human error and increases productivity. The intelligent design allows for temperature control and reliable sample injection, ensuring optimal results.

In terms of software, the 1100 Series is supported by Agilent’s OpenLAB software, which offers an intuitive platform for method development, data acquisition, and analysis. This software integrates seamlessly with the HPLC hardware, making it easier for scientists to manage workflows and maintain compliance with regulatory requirements.

Overall, the Agilent Technologies 1100 Series represents a significant advancement in liquid chromatography, bringing together ideal characteristics such as modularity, precision, versatility, and sophistication. Its commitment to innovation and quality makes it a preferred choice for laboratories seeking reliable analytical solutions.
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