Installing the Pump

Priming and Purging the System

Table 5

Choice of Priming Solvents for Different Purposes

 

 

 

 

 

 

Activity

Solvent

Comments

 

 

 

 

 

After an installation

Isopropanol

Best solvent to flush air out of

 

 

 

the system

 

When switching between

Isopropanol

Best solvent to flush air out of

 

reverse phase and normal

 

the system

 

phase (both times)

 

 

 

After an installation

Ethanol or Methanol

Alternative to Isopropanol

 

 

 

(second choice) if no

 

 

 

Isopropanol is available

 

To clean the system when

Bidistilled water

Best solvent to re-dissolve

 

using buffers

 

buffer cristals

 

After a solvent change

Bidistilled water

Best solvent to re-dissolve

 

 

 

buffer cristals

 

After the installation of

Hexane + 5% Isopropanol

Good wetting properties

 

normal phase seals (P/N

 

 

 

0905-1420)

 

 

 

 

 

 

30

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Agilent Technologies 1100 Series manual Activity Solvent Comments

1100 Series specifications

Agilent Technologies 1100 Series is a highly regarded lineup of high-performance liquid chromatography (HPLC) systems designed to meet the demanding needs of laboratories in various fields including pharmaceuticals, environmental testing, and food safety. Known for its versatility, reliability, and advanced technology, the 1100 Series has established itself as a benchmark for analytical performance.

One of the standout features of the Agilent 1100 Series is its modular architecture. This design allows users to configure the system according to specific analytical requirements. The components such as pumps, detectors, and autosamplers can be mixed and matched to create a tailored solution, ensuring maximum efficiency and optimal performance. This modularity also facilitates easy upgrades and maintenance, significantly extending the life cycle of the instrument.

At the heart of the 1100 Series is its advanced pumping technology. The system utilizes precise, high-pressure pumps that ensure a consistent flow rate and excellent reproducibility, essential for accurate quantification of analytes. The binary and quaternary pump options provide flexibility in method development, enabling users to perform gradient elution with ease.

The Agilent 1100 Series also boasts a range of detectors, including UV/VIS, fluorescence, and refractive index detectors. Each detector is designed to enhance sensitivity and specificity, enabling the detection of a wide array of compounds. The UV/VIS detector, in particular, is known for its reliable performance and user-friendly interface, which simplifies method setup and data analysis.

Another critical aspect of the 1100 Series is its autosampler. The system's autosamplers provide high throughput and automatic sample handling, which significantly reduces human error and increases productivity. The intelligent design allows for temperature control and reliable sample injection, ensuring optimal results.

In terms of software, the 1100 Series is supported by Agilent’s OpenLAB software, which offers an intuitive platform for method development, data acquisition, and analysis. This software integrates seamlessly with the HPLC hardware, making it easier for scientists to manage workflows and maintain compliance with regulatory requirements.

Overall, the Agilent Technologies 1100 Series represents a significant advancement in liquid chromatography, bringing together ideal characteristics such as modularity, precision, versatility, and sophistication. Its commitment to innovation and quality makes it a preferred choice for laboratories seeking reliable analytical solutions.