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Optimizing Performance

When to use the Continuous Seal Wash Option

When to use the Continuous Seal Wash Option

Highly-concentrated buffer solutions will reduce the lifetime of the seals and plungers in your quaternary pump. The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent.

The continuous seal wash option is strongly recommended when buffer concentrations of 0.1 Molar or higher will be used for long time periods in the quaternary pump.

The continuous seal wash option can be ordered by quoting part number 01018-68722 (kit contains parts for one pump head).

The seal wash option comprises a support ring, secondary seal, gasket and seal keeper for both plunger sides. A wash bottle filled with water /isopropanol (90/10) should be placed above the quaternary pump in the solvent cabinet and gravity will maintain a flow through the pump head removing all possible buffer crystals from the back of the pump seal.

Running dry is the worst case for a seal and drastically reduces its lifetime.

The seal will build up sticky layers on the surface of the plunger. These sticky layers will also reduce the lifetime of the primary seal. Therefore the tubes of the wash option should always be filled with solvent to prolong the lifetime of the wash seal. Always use a mixture of bidistilled water (90%) and isopropanol (10%) as wash solvent. This mixture prevents bacteria growth in the wash bottle and reduces the surface tension of the water. The flow rate should be regulated to approximately 20 drops/minute. This can be done with the velocity regulator supplied with the accessory kit.

For information on the installation of the continuous seal wash option refer to “Installing the Continuous Seal Wash Option” on page 116.

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Agilent Technologies 1100 Series manual When to use the Continuous Seal Wash Option
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1100 Series specifications

Agilent Technologies 1100 Series is a highly regarded lineup of high-performance liquid chromatography (HPLC) systems designed to meet the demanding needs of laboratories in various fields including pharmaceuticals, environmental testing, and food safety. Known for its versatility, reliability, and advanced technology, the 1100 Series has established itself as a benchmark for analytical performance.

One of the standout features of the Agilent 1100 Series is its modular architecture. This design allows users to configure the system according to specific analytical requirements. The components such as pumps, detectors, and autosamplers can be mixed and matched to create a tailored solution, ensuring maximum efficiency and optimal performance. This modularity also facilitates easy upgrades and maintenance, significantly extending the life cycle of the instrument.

At the heart of the 1100 Series is its advanced pumping technology. The system utilizes precise, high-pressure pumps that ensure a consistent flow rate and excellent reproducibility, essential for accurate quantification of analytes. The binary and quaternary pump options provide flexibility in method development, enabling users to perform gradient elution with ease.

The Agilent 1100 Series also boasts a range of detectors, including UV/VIS, fluorescence, and refractive index detectors. Each detector is designed to enhance sensitivity and specificity, enabling the detection of a wide array of compounds. The UV/VIS detector, in particular, is known for its reliable performance and user-friendly interface, which simplifies method setup and data analysis.

Another critical aspect of the 1100 Series is its autosampler. The system's autosamplers provide high throughput and automatic sample handling, which significantly reduces human error and increases productivity. The intelligent design allows for temperature control and reliable sample injection, ensuring optimal results.

In terms of software, the 1100 Series is supported by Agilent’s OpenLAB software, which offers an intuitive platform for method development, data acquisition, and analysis. This software integrates seamlessly with the HPLC hardware, making it easier for scientists to manage workflows and maintain compliance with regulatory requirements.

Overall, the Agilent Technologies 1100 Series represents a significant advancement in liquid chromatography, bringing together ideal characteristics such as modularity, precision, versatility, and sophistication. Its commitment to innovation and quality makes it a preferred choice for laboratories seeking reliable analytical solutions.
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