Advanced Operations

6.5 Multi-Wavelength mode – measurements at more than one wavelength

In the multi-wavelength mode, absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums, differences and relationships.

Absorbance measures the amount of light absorbed by the sample, in units of absorbance.

%Transmittance measures the percent of the original light that passes through the sample and reaches the detector.

Turning on the concentration factor allows selection of a specific multiplier for converting absorbance readings to concentration. In a graph of concentration versus the absorbance, the concentration factor is the slope of the line. Concentration is calculated using a single factor for each wavelength, which is input by the user.

6.5.1 Set the reading mode at different wavelengths

Press Multi Wavelength in the Main Menu. Press Options for

Parameter Setup.

 

Table 13 Multi-wavelength setup options

Options

Description

 

 

More

For further Options

 

 

Store Off/On

With the Store On setting, all measurement data are stored automatically. With the Store Off

setting, no measurement data are stored.

 

 

 

% Trans/Abs

To switch to % transmittance, concentration or absorbance readings

 

 

λ Wavelength

To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement

wavelength. The entered wavelength must be in the range from 340–900 nm.

 

 

 

 

This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed

Timer icon

(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has

elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement

 

 

program.

 

 

Concentration Factor

Multiplication factor for converting absorbance values into concentration values.

 

 

Concentration

To select the position of the decimal point in the calculated concentration readings.

Resolution

 

 

 

Absorbance Formula

Calculation basis for evaluating samples

 

 

Save as User Program

To store the selected parameters as a User Program, see section 6.1 on page 61

 

 

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Hach DR 2800 user manual Set the reading mode at different wavelengths, Multi-wavelength setup options