Leica Leica VT1200S manual Blade Method, Blade Forward back

5.Working with the Instrument

5.2.2 Fine-adjusting the Blade-to-Specimen proximity and Feeding to the Section Thickness

The rotary knob 2 for moving the specimen receptacle vertically can be used both for fine-adjusting the blade-to-specimen proximity and for adjusting the desired section thicknesses. The step sizes 1, 10 or 100μm can be se- lected by pressing the STEP SIZE key.

Turning the setting dial clockwise carries out the desired feed; turning the setting dial counterclockwise lowers the specimen (minus sign). The value appears in the lower display when "μm" is selected. After each sectioning process has been completed, the μm display is set to zero.

Save a commonly used section thickness by adjusting the setting dial for the section thickness and pressing the MEMO key for 3 seconds. An audi- ble signal confirms that the value has been accepted.

Feed to the saved value by quickly pressing the MEMO key. Pressing the MEMO multiple times feeds to the value multiple times.

Neither negative values nor a section thickness of over 1000μm are permitted. If an attempt is made to save impermissible values, an audible warning signal sounds three times and the last (permis- sible) value is retained.

5.2.3 Blade Method

The "Blade forward" and "Blade back" keys have to be held down until the desired position is reached. The blade feed rate is 2.5 mm/s. When each end point has been reached, the corresponding LED lights up in the key.

Blade

forward back

20 Operating Manual V1.1 – 05/2007