5Operating the Pump Motor in the STOPPED Display Mode
10.After you have finished dispensing, press the START/STOP key to stop the pump motor. Then measure the dispensed fluid’s volume using your calibrated beaker.
11.When you have determined the volume of the dispensed fluid, use the UP and DOWN ARROW keys to enter this volume on the display.
12.Once the display shows the correct volume of fluid dispensed, press the CALIBRATE key to complete the calibration. The display flashes and shows, “CALIBRATED!”, and the red LED next to the CALIBRATE key lights up.
13.Now you can select a mode (RPM, FLOW RATE, TIME or TOTAL VOLUME) (refer to Chapter 6). Once started, the fluid will be pumped according to the calibration.
NOTE
If you replace your tubing, make sure to press the TUBE ID key and select a different tube id size using the UP or DOWN ARROW keys.
NOTE
You can independently calibrate only one pump at a time. If you have two pumps attached to the motor (even if they are stacked), only one of the pumps can be calibrated.
For optimum FLOW RATE or TOTAL VOLUME accuracy, you will need to recalibrate the tubing as a result of:
•Using a different tube size (inner diameter)
•Using a different tubing material
•Using a tube of a different age (calibration should be checked every
•Using a different back pressure in the tube
•Changing the fluid being pumped
•Changing the fluid’s temperature or density
•Changing the RPM or FLOW RATE setting
•Reversing the direction of pumping
•Changing the clamp screw adjustment of the pump(s)
TIPS FOR OPTIMUM CALIBRATION ACCURACY:
Presented below are three useful techniques that will help you obtain optimum calibration accuracy.
•Calibrate the unit at the speed you plan to use for normal pump operation.
•When dispensing fluid during calibration, allow the pump to dispense the largest total volume that your measurement equipment supports.
•When measuring the dispensed fluid during calibration, calculate the volume by measuring the fluid's mass with an accurate gram scale and dividing this mass by the fluid's density.
