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Projection Television
Leica
CM3050 S instruction manual
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66 pages, 2.37 Mb
Contents
Main
Page
Important note
Table of Contents
Table of Contents
Table of contents
The chapters of the Leica CM3050 S cryostat instruction manual:
1. Important information
their meaning
1.1 Symbols used in this manual and
(5) (Fig.5)
General
1. Important information
1.2 Designated use
1.3 Qualification of personnel
2. Sicherheit
2.1 General information on instrument design and safe handling
2.2 Integrated safety devices
10
Unlocking:
2.2.2 Centering the handwheel grip
2.2.1 Locking the handwheel
Locking:
Page
12
2.3 Safety instructions: handling and operation of the instrument 2.3.1 Transport
2.3.2 Site requirements
2.3.3 Electrical connections
2.3.4 Handling microtome knives/blades
2.3.5 Knife guard/handwheel lock
2.3.6 Motorized sectioning
2.3.7 Defrosting/Handling frozen tissue
2.3.8 Frozen parts of the instrument and frozen accessories
14
2.3.9 Infectious/radioactive material
2.3.10 Disinfection and cleaning
2.3.11Removing/reinstalling the microtome
2.3.12 Display message Dry microtome
2.3.13 Maintenance
16
3.2 Unpacking and installation
3.2.1 Repacking
Available models
3.1 Site requirements
3.3 Standard delivery
18
Further accessories:
3.4 Installing/inserting accessories 3.4.1 Installing the handwheel
3.4.2 Inserting the accessories
20
3.4.3 The footswitch
Function
Models with footswitch:
3.5 Prior to switching on the instrument
4. Instrument Properties
4.1 Overview
Page
4. Instrument properties
4.2 Technical Data
4. Instrument properties
5.1 Operating the instrument for the first time
Page
5.3.1 Key functions in control panel 1
5.3 Control panel 1
5.3.2 Display functions in control panel 1
28
Meaning of indication: Operator activity:
5.3.3 Display indications when switching on
1. Specimen temperature: (instruments with specimen cooling only)
5.3.4 Menu functions: setting parameters of refrigeration, time and preset counter
30
2. Cryochamber temperature:
3. Preset counter (instruments with sectioning motor only):
7. Duration of defrost cycle:
4. Quick-freezing at maximum specimen temperature* (instruments with specimen cooling only)
5. Real time
6. Starting time of automatic defrost cycle:
32
5.3.5 Status display
HOME -
HOME
Each individual parameter is displayed in a certain area of the display screen.
5.3.6 Indication of refrigeration state, section thickness setting and counters
34
5.4 Control panel 2
Instruments without sectioning motor Instruments with sectioning motor
A B C
B
Start / stop motorized sectioning
5.4.1 Selecting a sectioning mode /
start and stop sectioning
Activating a sectioning mode
36
5.4.2 Setting the sectioning window
5.4.3 Selecting sectioning speed
5.4.4 Emergency stop
38
Functional area B - all models
5.4.5 Coarse feed keys
Selecting trimming thickness
Motorized trimming
5.4.7 Setting the section thickness
5.4.6 Trimming and sectioning functions
Functional area B - all models Switching between trimming and sectioning
40
5.5 Daily operation of the instrument
5.5.1 Freezing specimens onto specimen
discs with quick-freeze shelf and stationary heat extractor
5.5.2 Freezing specimens onto specimen
Installing the 90 prism
Freezing specimens with the 90 prism
discs with 90 prism (Instruments w/specimen cooling only)
5.5.3 Optional accessories for freezing Mobile heat extractor
42
5.5.4 Inserting specimen discs into the
specimen head
5.5.5 Specimen orientation
Thermoblock
Inserting the knife
5.5.6 Trimming / sectioning
Manual trimming
Motorized trimming
Sectioning
44
5.6 Finishing work 5.6.1 Daily routine steps
5.6.2 Switching off the instrument for a
longer period of time
5.7 Defrosting
5.7.1 Automatic chamber defrost cycle
Instruments with specimen cooling:
46
5.7.2 Manual chamber defrost cycle
Instruments with specimen cooling:
5.7.3 Defrosting the specimen head
(instruments with specimen cooling only)
6.1 Displayed error messages
1. Power failure
2. Coarse feed
3. Temperature indications
4. Service
48
6.2 Potential problems - causes and remedies
Page
Page
Page
52
6.3 Reference chart for temperature settings (in minus C)
Tissue 10 - 15 15 - 25 25 - 50
7.1 Safety instructions
7.2 Spray disinfection with Leica Cryofect
on disinfection and cleaning
54
7 Disinfection, cleaning and maintenance
disinfectants
7.3 Disinfection with conventional
7.4 Cleaning
7.5 Removing / reinstalling the microtome 7.5.1 Removing the microtome
7.5.2 Disassembling the specimen head
(instruments w/specimen cooling only)
56
7.5.3 Reinstalling the microtome
7
9
8
Page
8. Warranty and service
1.1 Designated use
1.2 Standard delivery
1.3 Safety instructions1. Electrohydraulic height adjustment
60
1.4 Installation
1.6 Maintenance and care
1.5 Control keys 1.7 Troubleshooting
1.8 Technical Data
62
2. Ordering information - accessories
2.1 Knife holders
2.2 Specimen discs
2.2 Accessories
EC Declaration of Conformity
11. Decontamination Certificate
A
B
1
3
2
11. Decontamination Certificate