5.Operation

5.5 Daily operation of the instrument

Select chamber temperature and, in instru- ments with specimen cooling, specimen temperature according to the type of speci- men to be sectioned (see also chapter 6.3 ‘Reference chart for temperature settings’).

Do not continue with step 5.5.1 before the actual chamber / (specimen) temperature corresponds to the set value. This may take several hours (e.g. when operating the in- strument for the first time or when the instru- ment had been switched off).

Prior to operating the instrument , please read and observe the fol- lowing safety instructions:

‘Integrated safety devices’ (Chapter 2.2) .

‘Handling microtome knives

/blades’ (Chapter 2.3.4)

‘Knife guard / Handwheel lock’ (Chapter 2.3.5)

’Motorized sectioning’ (Chapter 2.3.6)

‘Defrosting

/handling frozen tissue’ (Chapter 2.3.7)

‘Frozen instrument parts and accessories’ (Chapter 2.3.8)

‘Infectious / radioactive material’ (Chapter 2.3.9)

5.5.1Freezing specimens onto specimen discs with quick-freeze shelf and stationary heat extractor

Size the specimen.

Apply sufficient amount of embedding medi- um onto ambient temperature specimen disc.

Inside the cryochamber, place the specimen onto the disc and orient.

Place specimen disc with specimen into one of the 10 openings in the quick-freeze shelf (see below) and freeze.

2

 

 

 

1

 

 

 

3

The freezing process can be sped up further by using the stationary heat extractor (1). Next to the low temperature stabilizer (2) (‘Parking station’), 3 specimen discs (3) can be placed within its pivoting range.

Place the heat extractor (1) directly onto the specimen surface. After approx. 30 seconds the specimen is completely frozen.

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Instruction Manual V1.2 - 11/2006

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Leica CM3050 S instruction manual Daily operation of the instrument, ‘Knife guard / Handwheel lock’ Chapter