Leica CM3050 S | Leica instruction

Leica CM3050 S

Cryostat

Instruction Manual

Leica CM3050 S – Cryostat

English V1.2– 11/2006

Always keep this manual near the instrument.

Read carefully prior to operating the instrument.

Image 1

Leica instruction manual Leica CM3050 S

Contents

Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Important information Symbols used in this manual and their meaningSerial- number Designated use GeneralQualification of personnel Any other use of the instrument is considered improper Sicherheit Integrated safety devices Locking the handwheel SafetyLocking Unlocking Deactivating the emergency stop Activating the emergency stop functionKnife guard Electrical connections Site requirements Frozen parts of the instrument and frozen accessories Defrosting/Handling frozen tissueHandling microtome knives/blades Knife guard/handwheel lock Infectious/radioactive material Disinfection and cleaning Maintenance 11Removing/reinstalling the microtomeDisplay message ‘Dry microtome’ Unpacking and installation InstallationGeneral site requirements Repacking Standard delivery Further accessories Inserting the accessories Models with footswitch Footswitch FunctionConnecting the footswitch Prior to switching on the instrument Overview Instrument Properties Instrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time Operation Switching on Switching on/fusesMains switch / automatic cutout for the instrument Automatic cutout for sectioning motor Display functions in control panel Control panel Key functions in control panelLock / unlock key Activates / deactivates a number of functions Y M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling only Preset counter Instruments with sectioning motor only Cryochamber temperaturePress key until menu item ‘Preset counter’ is displayed = Preset counter activated Starting time of automatic defrost cycle Real timeDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFF Stop Status display Refrigeration state These parameters can be displayed via the ar- row keysSection thickness Total section thickness counter Instruments with sectioning motor Control panel Instruments without sectioning motor Sectioning mode 1 Intermittent stroke Activating a sectioning modeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Coarse feed ‘fast forward’ Travelling speed 1,000 μm/s Functional area B all models Coarse feed keysTo start, press and hold Selecting trimming thickness Setting the section thicknessMotorized trimming Daily operation of the instrument ‘Defrosting Handling frozen tissue’ Chapter‘Knife guard / Handwheel lock’ Chapter Freezing specimens with the 90 prism Installing the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ item Inserting specimen discs into the specimen head ThermoblockSpecimen orientation Manual trimming Trimming / sectioning Inserting the knifeSectioning Insert the knife / disposable blade into the knife holder Finishing work 5.6.1 Daily routine steps Switching off the instrument for a longer period of timeLock handwheel Automatic chamber defrost cycle DefrostingInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem Causes Problem Causes Luke-warm specimen disc Microtome defective Problem Causes Remedies Tissue Reference chart for temperature settings in minus C Safety instructions On disinfection and cleaning Disinfection, cleaning and maintenanceSpray disinfection with Leica Cryofect Allow to soak for 15 minutes Switch the instrument off CleaningDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it ‘Disinfection and cleaning’ Chapter Place plastic coupling 11 on handwheel coupling Reinstalling the microtomeUnlock handwheel Exchanging the fluorescent light lamp Installing the replacement lampRemoval Removing the sliding window Warranty Warranty and serviceTechnical service information Decommissioning and disposal Safety instructions Electrohydraulic height adjustmentAppendix Installation Troubleshooting Maintenance and careControl keys Accessories Ordering information accessories Knife holdersSpecimen discs Consumables Dag Graupner Managing Director EC Declaration of Conformity Decontamination Certificate master This equipment has been cleaned and decontaminatedDear Customer NB Microtome knives must be in boxes BU Return Authorisation Number