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Leica CM3050 S 52 , 6.3 Reference chart for temperature settings (in minus C)

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52 Instruction Manual V1.1 02/2000

6. Troubleshooting, applications tips

6.3 Reference chart for temperature settings (in minus °C)

Tissue 10 - 15 15 - 25 25 - 50

Adrenal 

Bone marrow ❄

Brain 

Bladder 

Breast - fatty 

Breast - little fat 

Cartilage 

Cervical 

Fatty 

Heart and vascular 

Intestinal 

Kidney 

Laryngeal 

Lip 

Liver 

Lung 

Lymphoid 

Muscular 

Nose 

Pancreatic 

Prostate 

Ovarian 

Rectal 

Skin with fat 

Skin without fat 

Spleenal or bloody tissue 

Testicular 

Thyroid 

Tongue 

Uterus curettage 

Please note: The recommended temperature settings are based on long-term experience. However, they can only be

guidelines for general orientation, as each individual specimen may require particular adjustments.

Contents

Main Page Important note Table of Contents Table of Contents Table of contents The chapters of the Leica CM3050 S cryostat instruction manual: 1. Important information their meaning 1.1 Symbols used in this manual and (5) (Fig.5) General 1. Important information 1.2 Designated use 1.3 Qualification of personnel 2. Sicherheit 2.1 General information on instrument design and safe handling 2.2 Integrated safety devices 10 Unlocking: 2.2.2 Centering the handwheel grip 2.2.1 Locking the handwheel Locking: Page 12 2.3 Safety instructions: handling and operation of the instrument 2.3.1 Transport 2.3.2 Site requirements 2.3.3 Electrical connections 2.3.4 Handling microtome knives/blades 2.3.5 Knife guard/handwheel lock 2.3.6 Motorized sectioning 2.3.7 Defrosting/Handling frozen tissue 2.3.8 Frozen parts of the instrument and frozen accessories 14 2.3.9 Infectious/radioactive material 2.3.10 Disinfection and cleaning 2.3.11Removing/reinstalling the microtome 2.3.12 Display message Dry microtome 2.3.13 Maintenance 16 3.2 Unpacking and installation 3.2.1 Repacking Available models 3.1 Site requirements 3.3 Standard delivery 18 Further accessories: 3.4 Installing/inserting accessories 3.4.1 Installing the handwheel 3.4.2 Inserting the accessories 20 3.4.3 The footswitch Function Models with footswitch: 3.5 Prior to switching on the instrument 4. Instrument Properties 4.1 Overview Page 4. Instrument properties 4.2 Technical Data 4. Instrument properties 5.1 Operating the instrument for the first time Page 5.3.1 Key functions in control panel 1 5.3 Control panel 1 5.3.2 Display functions in control panel 1 28 Meaning of indication: Operator activity: 5.3.3 Display indications when switching on 1. Specimen temperature: (instruments with specimen cooling only) 5.3.4 Menu functions: setting parameters of refrigeration, time and preset counter 30 2. Cryochamber temperature: 3. Preset counter (instruments with sectioning motor only): 7. Duration of defrost cycle: 4. Quick-freezing at maximum specimen temperature* (instruments with specimen cooling only) 5. Real time 6. Starting time of automatic defrost cycle: 32 5.3.5 Status display HOME - HOME Each individual parameter is displayed in a certain area of the display screen. 5.3.6 Indication of refrigeration state, section thickness setting and counters 34 5.4 Control panel 2 Instruments without sectioning motor Instruments with sectioning motor A B C B Start / stop motorized sectioning 5.4.1 Selecting a sectioning mode / start and stop sectioning Activating a sectioning mode 36 5.4.2 Setting the sectioning window 5.4.3 Selecting sectioning speed 5.4.4 Emergency stop 38 Functional area B - all models 5.4.5 Coarse feed keys Selecting trimming thickness Motorized trimming 5.4.7 Setting the section thickness 5.4.6 Trimming and sectioning functions Functional area B - all models Switching between trimming and sectioning 40 5.5 Daily operation of the instrument 5.5.1 Freezing specimens onto specimen discs with quick-freeze shelf and stationary heat extractor 5.5.2 Freezing specimens onto specimen Installing the 90 prism Freezing specimens with the 90 prism discs with 90 prism (Instruments w/specimen cooling only) 5.5.3 Optional accessories for freezing Mobile heat extractor 42 5.5.4 Inserting specimen discs into the specimen head 5.5.5 Specimen orientation Thermoblock Inserting the knife 5.5.6 Trimming / sectioning Manual trimming Motorized trimming Sectioning 44 5.6 Finishing work 5.6.1 Daily routine steps 5.6.2 Switching off the instrument for a longer period of time 5.7 Defrosting 5.7.1 Automatic chamber defrost cycle Instruments with specimen cooling: 46 5.7.2 Manual chamber defrost cycle Instruments with specimen cooling: 5.7.3 Defrosting the specimen head (instruments with specimen cooling only) 6.1 Displayed error messages 1. Power failure 2. Coarse feed 3. Temperature indications 4. Service 48 6.2 Potential problems - causes and remedies Page Page Page 52 6.3 Reference chart for temperature settings (in minus C) Tissue 10 - 15 15 - 25 25 - 50 7.1 Safety instructions 7.2 Spray disinfection with Leica Cryofect on disinfection and cleaning 54 7 Disinfection, cleaning and maintenance disinfectants 7.3 Disinfection with conventional 7.4 Cleaning 7.5 Removing / reinstalling the microtome 7.5.1 Removing the microtome 7.5.2 Disassembling the specimen head (instruments w/specimen cooling only) 56 7.5.3 Reinstalling the microtome 7 9 8 Page 8. Warranty and service 1.1 Designated use 1.2 Standard delivery 1.3 Safety instructions1. Electrohydraulic height adjustment 60 1.4 Installation 1.6 Maintenance and care 1.5 Control keys 1.7 Troubleshooting 1.8 Technical Data 62 2. Ordering information - accessories 2.1 Knife holders 2.2 Specimen discs 2.2 Accessories EC Declaration of Conformity 11. Decontamination Certificate A B 1 3 2 11. Decontamination Certificate