Instrument Properties, Overview | Leica CM3050 S instruction

4.Instrument Properties

4.1 Overview

1Cryostat CM3050S

2 Control panel 1

3 Control panel 2

4 Storage shelf, left

5 Storage shelf, right

6 Rubber mat

7 Mains switch / Automatic cutout for instrument, Automatic cutout for sectioning motor, Footswitch port

8 Quick-freeze shelf

9 Stationary heat extractor

10 Mobile heat extractor

11 Specimen disc

12 Thermoblock (optional)

13 Section waste tray

14 Brush shelf

15 Specimen head w/o specimen cooling

16 Specimen head with specimen cooling (Option)

17 90° Prism (instruments with specimen cooling only)

18 Knife holder base

19 Knife holder CE

20 Knife holder CN

21 Knife holder CS

22 Footswitch with protective guard

Leica CM3050S – Cryostat

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Leica CM3050 S instruction manual Instrument Properties, Overview

Contents

Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Symbols used in this manual and their meaning Important informationSerial- number Designated use GeneralQualification of personnel Any other use of the instrument is considered improper Sicherheit Integrated safety devices Locking the handwheel SafetyLocking Unlocking Activating the emergency stop function Deactivating the emergency stopKnife guard Electrical connections Site requirements Frozen parts of the instrument and frozen accessories Defrosting/Handling frozen tissueHandling microtome knives/blades Knife guard/handwheel lock Infectious/radioactive material Disinfection and cleaning 11Removing/reinstalling the microtome MaintenanceDisplay message ‘Dry microtome’ Unpacking and installation InstallationGeneral site requirements Repacking Standard delivery Further accessories Inserting the accessories Models with footswitch Footswitch FunctionConnecting the footswitch Prior to switching on the instrument Overview Instrument Properties Instrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time Operation Switching on Switching on/fusesMains switch / automatic cutout for the instrument Automatic cutout for sectioning motor Display functions in control panel Control panel Key functions in control panelLock / unlock key Activates / deactivates a number of functions Y M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling only Preset counter Instruments with sectioning motor only Cryochamber temperaturePress key until menu item ‘Preset counter’ is displayed = Preset counter activated Starting time of automatic defrost cycle Real timeDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFF Stop Status display These parameters can be displayed via the ar- row keys Refrigeration stateSection thickness Total section thickness counter Instruments with sectioning motor Control panel Instruments without sectioning motor Activating a sectioning mode Sectioning mode 1 Intermittent strokeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Functional area B all models Coarse feed keys Coarse feed ‘fast forward’ Travelling speed 1,000 μm/sTo start, press and hold Setting the section thickness Selecting trimming thicknessMotorized trimming ‘Defrosting Handling frozen tissue’ Chapter Daily operation of the instrument‘Knife guard / Handwheel lock’ Chapter Freezing specimens with the 90 prism Installing the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ item Thermoblock Inserting specimen discs into the specimen headSpecimen orientation Manual trimming Trimming / sectioning Inserting the knifeSectioning Insert the knife / disposable blade into the knife holder Switching off the instrument for a longer period of time Finishing work 5.6.1 Daily routine stepsLock handwheel Defrosting Automatic chamber defrost cycleInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem Causes Problem Causes Luke-warm specimen disc Microtome defective Problem Causes Remedies Tissue Reference chart for temperature settings in minus C Safety instructions On disinfection and cleaning Disinfection, cleaning and maintenanceSpray disinfection with Leica Cryofect Allow to soak for 15 minutes Switch the instrument off CleaningDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it ‘Disinfection and cleaning’ Chapter Reinstalling the microtome Place plastic coupling 11 on handwheel couplingUnlock handwheel Exchanging the fluorescent light lamp Installing the replacement lampRemoval Removing the sliding window Warranty Warranty and serviceTechnical service information Decommissioning and disposal Electrohydraulic height adjustment Safety instructionsAppendix Installation Maintenance and care TroubleshootingControl keys Accessories Ordering information accessories Knife holdersSpecimen discs Consumables Dag Graupner Managing Director EC Declaration of Conformity Decontamination Certificate master This equipment has been cleaned and decontaminatedDear Customer NB Microtome knives must be in boxes BU Return Authorisation Number