5.Operation

Thermoblock

The thermoblock makes it easy to remove fro- zen specimens from the specimen discs.

Do not store the thermoblock inside the cryochamber - store at room temperature and only place into the chamber for removing specimens from discs.

Place cap (9) on one of the two ends so that the correct size opening (7) for the disc to be inserted is exposed.

Insert pin (6) of specimen disc (5) into the opening (7).

After about 20 seconds remove the frozen specimen from the specimen disc (use tweezers).

If cap (9) fits too loosely, readjust with screw (10). Do not tighten screw too much!

Once the specimen has been detached from the disc, remove the thermoblock from the cryochamber.

5.5.4Inserting specimen discs into the specimen head

• Lock handwheel in upper position.

If a knife has already been inserted into the knife holder, cover knife edge with knife guard!

Loosen clamping screw (3).

Insert specimen disc in opening (2) (rear surface of specimen disc must fit tightly against surface of specimen head).

Tighten clamping screw (3).

5.5.5 Specimen orientation

Open clamping lever (4) --> (4a).

Orient specimen via orienting screws (1) and (5).

Retighten clamping lever (4).

42

Instruction Manual V1.2 - 11/2006

Page 42
Image 42
Leica CM3050 S instruction manual Thermoblock, Inserting specimen discs into the specimen head, Specimen orientation