7.Disinfection, cleaning and maintenance

7.1Safety instructions

on disinfection and cleaning

When disinfecting the instrument, make sure to observe the following safety instructions:

‘Disinfection and Cleaning’ (Chapter 2.3.10)

‘Handling microtome knives / blades’

(Chapter 2.3.4)

‘Defrosting / handling frozen tissue’

(Chapter 2.3.7)

‘Frozen instrument parts and accessories’

(Chapter 2.3.8)

‘Infectious / radioactive material’ (Chapter 2.3.9)

7.2 Spray disinfection with Leica Cryofect

For practical spray disinfection, we recommend Leica Cryofect which is fully effective at tem- peratures in the negative range down to -20 °C.

Set chamber temperature -20 °C or warmer.

Remove specimens from the chamber and store safely.

Carefully remove knife / blade from knife holder.

Remove section waste tray from cryocham- ber and empty.

Remove remaining section waste from cryo- chamber.

Working accessories (brushes, specimen holders etc.) can be disinfected inside the cryochamber.

Once the selected chamber temperature has been reached, spray Leica Cryofect on the surfaces and accessories to be disinfected.

Allow to soak for 15 minutes.

Use paper towels or another type of disposable cleaning cloths to wipe up disinfectant and remaining section waste.

Dispose of paper towels together with sec- tion waste.

If previously altered, set chamber tempera- ture back to its former value.

Put specimens back into the cryochamber.

Leica CM 3050 S – Cryostat

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Leica CM3050 S instruction manual Disinfection, cleaning and maintenance, Safety instructions On disinfection and cleaning