Leica CM3050 S instruction

66	Instruction Manual V1.2 - 11/2006

Image 66

Leica CM3050 S instruction manual

Contents

Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Symbols used in this manual and their meaning Important informationSerial- number Qualification of personnel GeneralDesignated use Any other use of the instrument is considered improper Integrated safety devices Sicherheit Locking SafetyLocking the handwheel Unlocking Activating the emergency stop function Deactivating the emergency stopKnife guard Site requirements Electrical connections Handling microtome knives/blades Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Knife guard/handwheel lock Disinfection and cleaning Infectious/radioactive material 11Removing/reinstalling the microtome MaintenanceDisplay message ‘Dry microtome’ General site requirements InstallationUnpacking and installation Repacking Standard delivery Further accessories Inserting the accessories Connecting the footswitch Footswitch FunctionModels with footswitch Prior to switching on the instrument Instrument Properties Overview Instrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first time Mains switch / automatic cutout for the instrument Switching on/fusesSwitching on Automatic cutout for sectioning motor Lock / unlock key Control panel Key functions in control panelDisplay functions in control panel Activates / deactivates a number of functions Y M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute steps Press key until menu item ‘Preset counter’ is displayed Cryochamber temperaturePreset counter Instruments with sectioning motor only = Preset counter activated Duration of defrost cycle Real timeStarting time of automatic defrost cycle Press lock/unlock key to activate or de- activate ON/OFF Status display Stop These parameters can be displayed via the ar- row keys Refrigeration stateSection thickness Total section thickness counter Control panel Instruments without sectioning motor Instruments with sectioning motor Activating a sectioning mode Sectioning mode 1 Intermittent strokeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Functional area B all models Coarse feed keys Coarse feed ‘fast forward’ Travelling speed 1,000 μm/sTo start, press and hold Setting the section thickness Selecting trimming thicknessMotorized trimming ‘Defrosting Handling frozen tissue’ Chapter Daily operation of the instrument‘Knife guard / Handwheel lock’ Chapter Mobile heat extractor Installing the 90 prismFreezing specimens with the 90 prism See .3.4 ‘Menu functions’ item Thermoblock Inserting specimen discs into the specimen headSpecimen orientation Sectioning Trimming / sectioning Inserting the knifeManual trimming Insert the knife / disposable blade into the knife holder Switching off the instrument for a longer period of time Finishing work 5.6.1 Daily routine stepsLock handwheel Defrosting Automatic chamber defrost cycleInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes Remedies Problem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defective Reference chart for temperature settings in minus C Tissue Spray disinfection with Leica Cryofect Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Allow to soak for 15 minutes Disinfection with conventional disinfectants CleaningSwitch the instrument off Remove section waste from the chamber and dispose of it ‘Disinfection and cleaning’ Chapter Reinstalling the microtome Place plastic coupling 11 on handwheel couplingUnlock handwheel Removal Installing the replacement lampExchanging the fluorescent light lamp Removing the sliding window Technical service information Warranty and serviceWarranty Decommissioning and disposal Electrohydraulic height adjustment Safety instructionsAppendix Installation Maintenance and care TroubleshootingControl keys Specimen discs Ordering information accessories Knife holdersAccessories Consumables EC Declaration of Conformity Dag Graupner Managing Director Dear Customer This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxes BU Return Authorisation Number