Leica DM1000 LED manual Checking Phase Contrast Rings

7. Start-up

•Using the condenser height adjuster (25.1), adjust the condenser until the edge of the field diaphragm appears in sharp relief (26b)

•If the image does not appear in the middle of the field of view (26c), the condenser must be moved into the middle of the field of view with the help of the two centering bolts (25.4); the tool required for this purpose is magnetically attached to the underside of the stage

•Open the field diaphragm just enough for it to disappear from the field of view (26d)

Note:

The condenser height adjustment depends on the thickness of the specimen. It may be adjusted for different specimens.

Fig. 25

1Condenser height adjuster

2Brightness control

3Field diaphragm

4Condenser centering

7.3 Checking Phase Contrast Rings

If your microscope is equipped for the use of phase contrast, the light rings that fit the objec- tives are built into the condenser disk*.

The light rings are already centered in the factory. However, the centering should be rechecked.

Note:

A light ring slide which is inserted into the side of the condenser is used for condensers without condenser disks. Centering is not required in this case.

Note:

When swivelling in a suitable objective for phase contrast, the corresponding light ring must be chosen.

The objective engraving (e.g. PH 1) indicates the corresponding light ring (e.g. 1).

Fig. 26 Köhler illumination

aField diaphragm not focused, not centered

bField diaphragm focused, but not centered

cField diaphragm focused and centered, diameter is too small, however

dField diameter (light) = Field diameter (view) (Köhler illumination)

4

ab

cd

1 2 3

29