Leica DM1000 LED Contrast Methods Phase Contrast, Use the light ring slide, Darkfield, 44 OIL

9. Contrast Methods

9.1.2 Phase Contrast*

•Insert a transmitted light specimen

•Rotate an appropriate objective into place; objectives that are suitable for phase contrast are engraved with PH

•Bring the image into focus using the focus dial and set the brightness

•For an optimal field diaphragm setting, check the Köhler illumination (→ p. 28)

•Open the aperture diaphragm completely (position PH)

•Condensers UCL/UCLP and UCA/P:

Set the light ring corresponding to the objective on the condenser disk

Example: Light ring 1 belongs to the objective with the engraving PH 1

Condensers CL/PH, CLP/PH, and APL. ACHR.0.9 (P):

Use the light ring slide

9.1.3 Darkfield*

•Insert a transmitted light specimen

•Rotate an appropriate objective into place

•Bring the image into focus using the focus dial and set the brightness

•Condenser UCL/P:

Click the condenser disk into the BF position Condensers CL/PH, CLP/PH, and

APL .ACHR.0.9 (P):

Pull out the DF light ring slide as far as the stop;

check the Köhler illumination (→ p. 28)

•Open the aperture diaphragm completely (position PH)

•Condenser UCL/P:

Click the condenser disk into the DF position Condensers CL/PH, CLP/PH, and APL.ACHR.0.9 (P):

Insert the DF light ring slide as far as the stop

Note:Note:

Condensers UCL/UCLP and UCA/P: Light rings must be centered (→ p. 29).

Condensers UCL/UCLP and UCA/P: The DF light ring must be centered (→ p. 29).

Special dark field condensers* are available for the DM1000/DM1000 LED.

The application potential of the DF condensers depends on the aperture of the objective in use. For objectives with built-in iris diaphragm, the aperture can be adapted.

DF condenser		max. objective aperture
D 0.80	- 0.95	0.75
D 1.20	- 1.44 OIL	1.10

44