Leica DM1000 LED manual Contrast Methods

9. Contrast Methods

9.Contrast Methods

9.1Transmitted Light Objective Magnification 2.5 x*

The CL/PH and CLP/PH condensers can be used alone starting at 4x magnification.

When using a diffuser slider*, 2.5x magnifi- cation is also possible; not when using polari- zation, however.

The UCL and UCLP condensers can also be used alone starting at 4x magnification.

When using an adapter lens* (in the condenser disk), 2.5x magnification is also possible. Before using the adapter lens, set Köhler illumination (→ p. 28) with 4x or 10x objective. Switch over to objective 2.5x, engage the lens, open the aperture diaphragm as far as the stop and narrow the field diaphragm.

In case of arc-shaped vignetting, center the lens: insert both centering keys into the condenser at an angle from the back and adjust until the asymmetrical vignetting disappears. Remove the centering keys and open the field diaphragm.

The lens can only be used up to an objective magnification of max. 20x. Exact Köhler illumination can no longer be obtained!

The Achr.Apl.0.9 (P) condenser can be used alone starting at 4x magnification.

With the condenser head swung out, 2.5x objective magnification is possible without a dif- fuser; with the head swung in, the diffuser must be in place (max. eyepiece field number 22).

Objectives with magnifications < 10x are used with the condenser head folded out, magnifi- cations of 10x upwards (up to 100x) with the condenser head folded in.

With the use of the switchable polarizer (11 555

034)and by swinging-out the blue filter (11 505 210 or 11 505 211) you need to unscrew the outer longer condenser lever.

The optional diffuser* (11 505 219) resp. the condenser lens* (11 505 507) for lower objective magnifications (1.25x–5x) is placed with the opening on the condenser top. The diffuser/ condenser lens* will be automatically switched into the light path when the condenser top is swung out by use of the objective magnifi- cations lower than 10x.

Magnifications of 1.6x and 2.5x are also possible with the CL/PH or CLP/PH, UCL or UCLP condensers if the condenser is removed completely. The field diaphragm then takes over the function of the aperture diaphragm.

Note:

If the microscope is equipped for polarization, the analyzer and polarizer as well as the lambda plate compensator must be removed or swung out when using other contrast methods.

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