9. Contrast Methods

9.1.1 Brightfield

If present: click the condenser disk* into the BF position

If present: pull the light ring slide* out of the condenser

If present: switch the fluorescence illuminator into an empty position or filter system A

Insert a transmitted light specimen

Rotate an appropriate objective into place

Movable condenser heads:

The condenser top is swung out for objective magnifications < 10x

Bring the image into focus using the focus dial and set the brightness

For an optimal field diaphragm setting, check the Köhler illumination (p. 31)

Fig. 47 Filter holders*

DLF filter magazine for attachment to micro- scope base

• Use suitable transmitted-light filters as Filter holder with

applicable (fig. 47)

two positions or one po- sition for attachment to microscope base

Filter holder for screw attachment on the under- side of the condenser

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Leica DM1000 LED manual Contrast Methods Brightfield