Leica DM1000 LED Trouble shooting Problem Cause/Remedy Phase Contrast, Fluorescence is too weak

11. Trouble shooting

Problem Cause/Remedy

Phase Contrast

No phase contrast is possible.

￿The specimen is too thick, too thin or too brightly stained

￿Refractive index of mounting medium and specimen is identical so that there is no phase jump

￿The cover glass is not placed evenly ￿Check the right light ring (→ p. 44) ￿Check the centering of the light rings

(→ p. 29-30)

￿Check the condenser centering

￿Open the aperture diaphragm completely

Fluorescence

The image is completely dark (no fluorescence).	￿Open the shutter (→ p. 46)
	￿Check the antigen-antibody combination
	￿Insert a new lamp (→ p. 21 onwards)

The fluorescence is too weak.

Stage

￿Center the lamp (→ p. 31 onwards) ￿Insert a new lamp (→ p. 21 onwards)

Travel range of the stage in e-w direction decreases after a longer period of working.

￿Move the stage all the way to the left side with the coaxial pinion

￿Push one of the screws which hold the object guide with your hand further to the left as far as it will go

￿Afterwards move the stage all the way to the right

￿Push one of the screws which hold the object guide with your hand further to the right as far as it will go

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