Leica DM1000 LED manual Focus on the specimen with the focus wheel

7. Start-up

•In the place of an eyepiece, insert the focus- ing telescope (fig. 27) into the observation tube

•Swivel in the phase contrast objective with the lowest magnification

•Focus on the specimen with the focus wheel

•Focus the ring structure (29.a) by slightly loos- ening the clamping ring (27.2) and moving the eye lens (27.1)

•Turn the centering screws until the dark ring (phase ring in the objective) is congruent with the slightly narrower bright ring (light ring in condenser) (29 c)

•Repeat the process for all other light rings and objectives

•Optionally remove the centering keys after the centering procedure

Fig. 28 Light ring centering (i.e.: condenser UCL/P)

1Centering keys

• Retighten the clamping ring

1 1

• Select the corresponding ring diaphragm (light ring) in the condenser.

•If the light ring and the phase ring are not shown as arranged in fig. 29.c, the light ring must be centered

•Insert the centering screws into the openings provided at the rear of the condenser (28.1)

		Fig. 29 Phase contrast centering procedure
		PH=phase contrast ring, LR=light ring
		a Condenser in bright field (BF) position
Fig. 27 Focusing telescope		b Condenser in phase contrast (PH) position
1	Adjustable eye lens	Light ring (LR) not centered
2	Clamping ring for fixing the focus position	c Light ring and phase ring centered

a	b	c
1
2

PHLR

30