Leica DM1000 LED manual Contrast Methods Oblique Illumination, Polarization

9. Contrast Methods

9.1.4 Oblique Illumination*

•First adjust transmitted light darkfield

•To obtain a relief-like contrast: Condenser UCA/P:

Rotate condenser disk slightly out of the DF position

Condensers CL/PH, CLP/PH, and APL. ACHR.0.9 (P):

Push DF slide in part way out of the DF position

9.1.5 Polarization*

•Swing the lambda plate of the lambda plate compensator out if your microscope is equipped with it

•Insert a specimen and rotate an appropriate objective into place

•Bring the image into focus and set the Köhler illumination (→ p. 28)

•Depending on the equipment, the analyzer may already have been inserted into the tube mount during the assembly

Alternative:

If the analyzer mount TL* is used:

Insert the analyzer as far as the clickstop into the microscope stand. The engraving λ must be on the underside

When using the Pol intermediate tube*: Switch on the analyzer

•Push the polarizer with the labelled side upward into the lower opening

!Attention!

Always use the polarizer with the labelled side upward, as otherwise the integrated heat protection filter is ineffective and the special polarizer will become useless (discoloring!).

•Bring the polarizer and analyzer into cross po- sition until they reach maximum darkness

•Remove the object or find an empty area of the specimen

•Push the analyzer into the stand as far as the 2nd clickstop or switch on the module

•Remove compensators from the light path if your microscope is equipped with it

•Rotate the polarizer until you observe the maximum extinction position in the eyepiece (fig 48)

•Fix the cross position thus determined with the clamping screw

Fig. 48

Crossing the polarizers when observing through a focusing telescope or Bertrand lens, high-aperture Pol objective

aexactly crossed, b not exactly crossed

Pos. a cannot be set if there is strain in the condenser or objective, Pos. b is adequate for polarization contrast.

a b

45