8. Operation

8.7Light sources Transmitted light

Adjust the brightness with the dial (45.1).

The numbers on the dial are not absolute values, but are intended to enable reproducible settings.

Note:

 

The

 

HI PLAN xx SL*

and

HI PLAN CY xx SL*

(Synchronized Light) objective lines permit objectives to be changed without having to adjust the light intensity in addition.

Fluorescence*

8.8 Aperture Diaphragm

The aperture diaphragm (46.3) determines the resolution, depth of field and contast of the microscope image. The best resolution is obtained when the apertures of the objective and the condenser are roughly the same.

When the aperture diaphragm is stopped down to be smaller than the objective aperture, resolving power is reduced, but the contrast is enhanced. A noticeable reduction in the resolving power is observed when the aperture diaphragm is stopped down to less than 0.6x of the objective aperture and should be avoided where possible.

In polarization microscopy, stopping down the aperture diaphragm generally results in more intense colors.

Switch on the lamp at the external power unit.

Caution!

Keep the lamphousing at least 10 cm away from the wall, curtains, wallpaper, books and other combustible objects!

Fire Hazard!

The aperture diaphragm is set according to the viewer’s subjective impression of the image, the scale on the dial is just to allow reproducible settings and does not represent absolute aperture values.

Please read the separate documentation for the

Fig. 45

supply unit.

1 Brightness control

1

40

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Leica DM1000 LED manual Fluorescence Aperture Diaphragm, Fire Hazard, Supply unit
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