Leica DM1000 LED manual Contrast Methods, Fluorescence

9. Contrast Methods

•If present:

Insert the λ or λ/4 compensator* into the filter holder integrated in the condenser holder and rotate to the left, roughly as far as the stop CLP/PH condenser:

Insert the λ or λ/4 compensator into the slot on the side of the condenser.

Condensers UCLP and UCA/P:

Rotate the condenser disk into position λ or λ/4

9.2 Fluorescence*

•Insert a suitable specimen and rotate an appropriate objective into place

•Focus the image initially in transmitted light if appropriate

•Switch on the incident light source at the external power unit

•Switch off the transmitted light illumination

•Open the shutter

•Select an appropriate fluorescence filter cube

•Switch magnification changer*, if present, to factor 1x

•Disengage the BG 38 filter if there is no disturbing red background; always engage the filter for photography, however

Fig. 49 Leica DM1000 with

Fluorescence illuminator and 106z lamphousing

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