46
9. Contrast Methods
• If present:
Insert the λ or λ/4 compensator* into the filter
holder integrated in the condenser holder and
rotate to the left, roughly as far as the stop
CLP/PH condenser:
Insert the λ or λ/4 compensator into the slot
on the side of the condenser.
Condensers UCLP and UCA/P:
Rotate the condenser disk into position λ or
λ/4
9.2 Fluorescence*
• Insert a suitable specimen and rotate an
appropriate objective into place
• Focus the image initially in transmitted light if
appropriate
• Switch on the incident light source at the
external power unit
• Switch off the transmitted light illumination
• Open the shutter
• Select an appropriate fluorescence filter cube
• Switch magnification changer*, if present, to
factor 1x
• Disengage the BG 38 filter if there is no
disturbing red background; always engage
the filter for photography, however
Fig. 49 Leica DM1000 with
Fluorescence illuminator and 106z lamphousing