Leica TL RCI Relief Images, Starting position | Leica M205 FA

Leica TL RCI™: Relief Images

Starting position

1.Push the deflection mirror all the way back towards the column.

2.Turn the deflection mirror into the notch position at an angle of 45°.

Positive relief contrast

Suitable for semitransparent and transparent specimens. The phase structures look like hills.

The effect can be strengthened or weakened by gently tilting the deflection mirror.

Negative relief contrast

Suitable for semitransparent and transparent specimens. These settings result in a nega- tive relief contrast. Phase structures look like valleys.

The effect can be strengthened or weakened by gently tilting the deflection mirror.

Leica M-series	User Manual	100

Image 100

Leica M205 FA user manual Leica TL RCI Relief Images, Starting position, Positive relief contrast, Negative relief contrast

Contents

Leica M165 FC Leica M205 FA General Instructions Special manuals are provided for a number of accessories Important Safety NotesSpecial manuals Important information Symbols UsedExplanatory notes Safety Instructions DescriptionIntended use Non-intended use Safety Instructions contd Beam paths to protect the eyes Radiation from reaching the eyesUV radiation could damage the eyes. Therefore Supply unit Lamp housingMercury lamp Contents Objective Nosepiece Iris DiaphragmDifferent Types of Objectives 137 Care, Maintenance, Contact Persons 151150 Leica M Series for Fluorescence Congratulations Step Towards Infinity Limits are made to be broken Reliability for your experiments Fewer cables Modular Design Everything is Relative Have a special request? Let us know Maximum Compatibility ObjectivesTubes Eyepieces On We Go Assembly Tools used Assembling the Focusing Column for MDG BasesΡρ Hex socket screwdriver, 3 mm Assembling the Focusing Column with an Incident-light Base Assembly Readjusting the motorized focus Motorized Focus Restricting the Travel PathRestricting the bottom travel range Tighten the screw of the limit stop Optics Carrier Ρρ Hex socket screwdriver, 4 mm Tube Ρρ No tools requiredPreparations Assembling the tube Tools used Preparation EyepiecesMagnification range Attaching the objective ObjectiveAlternative fastening options Safety Notes UV Protection Screen AssemblyUse with one objective Objective Nosepiece Assembly Do not fall onto the stage plateRemove the transport anchor from the objective nosepiece Screw the optics carrier onto the objective nosepiece Adjustment Objective Nosepiece Adjusting ParfocalityTighten the locking screws Unpacking the base Focusing drive and column Below using the four hexagon-headTransmitted-light Base TL ST Base is delivered with the adapter plate FluoCombi III Preparations Screw the 5× planapochromat into the FluoCombi FluoCombi III Assembly FluoCombi III Assembly contd Assembling the optics carrier FluoCombi III Adjusting the Parfocality Setting up the objective nosepieceBe carried out once. It ensures that Under the beam path FluoCombi III Adjusting the Parfocality contd FluoCombi III Centering the Objectives Objective is changedEasier Centering the objective FluoCombi III Filter and UV Protection Screen Inserting the dichroic mirrorPull the dummy filter carrier out of the holder Assembling the UV protection screen Removing the transport anchors Lutely necessary to remove the two transport anchors Stage assembly Transmitted-light Base TL BfdfStandard delivery Standard stage TL RC / TL RCI Leica IsoPro Mechanical Stage IsoPro Manual Mechanical Stage AssemblyLeft or right operation Leica IsoPro Manual Mechanical Stage Assembly contd Control assemblyMechanical stage assembly Place the mechanical stage onto the base Insert the glass plate into the mechanical stage Leica IsoPro Motorized Mechanical Stage Assembly Basics Leica IsoPro Motorized Mechanical Stage Assembly contd Future transport Bases Mechanical stage and base Mechanical stage for X-Y stage control module Ρρ The interface to the optics carrier is on the column Cables ConnectionsTerminals Cables Cable Duct Feeding the cables Cables Diagram Alternative installation Ing stirrups on both top holes on the drive housing Leica LED5000 MCIHand and tightly screw the retain Constraints Installation on the column Leica LED5000 MCI Alternative AssemblyPull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 RL Leica EL6000 Safety Notes General safety notesRights to any warranty claims Such cases, the Declaration of Conformity shall be invalid Leica EL6000 Safety Notes contd Use the instrument in rooms with explosion hazard Electrical safety Setting up the instrument Instructions for lamp replacementLeica EL6000 Preparations Inserting the lamp Leica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Leica EL6000 Replacing the Lamp Replacing the lamp Leica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Adjusting the interpupillary distance Correct Interpupillary DistanceReference value Look into the eyepieces Using the Eyepieces Dioptric correctionIf you do not wear glasses If you wear glasses Focusing FocusingCoarse/fine adjustment Adjusting the resistance Adjusting the Resistance of the Focus DriveFocusing Zooming Changing Magnification ZoomLook into the eyepieces Focus on the specimen Enabling and disabling ratchet steps Ratchet Steps and Magnification LevelsMagnifications and fields of view Parfocality More Comfort and Convenience for Your Work Requirements for parfocal workParfocality Enlarge the view to the maximum level Focus on the specimen Closing the iris diaphragm Iris DiaphragmOpening the Iris Diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M series Health Notes Potential sources of infection Using the Dioptric Correction Dioptric Correction Adjusting Dioptric Correction and Parfocality Graticules UseUse with the Leica AX carrier Convergence angle in the stereoscopic image Photography & Video Photography & Video AdapterLeica DFC cameras Leica Application Suite Photo Tubes and C-mounts Trinocular Video/Phototube 50% Photo beam path Switchover Trinocular Video/Phototube 100% Microscope Carrier Objective Nosepiece Parfocal workObjective change Technical constraints Objectives Optical Accessories Different Types of Objectives Achromatic objectivesPlanachromatic objective 1× Achromatic objectives with a long focal length Bases Leica TL ST Transmitted-light Base Controls Leica TL ST Transmitted-light Base Operation Light intensity controlTransmitted-light control Bright field Leica TL ST Transmitted-light Base Changing Bulbs Precautionary measuresChanging the halogen lamp Adapter at the focusing drive Leica TL Bfdf Transmitted-light Base Controls Manufacturers of the light guide and cold light source Leica TL Bfdf Transmitted-light Base OperationDark field Leica TL RC / TL RCI Controls Leica TL RCI The Deflection Mirror Inverted world?Tilted mirror Functions of the rotary knob Leica TL RCI Color Intensity and Temperature Using a USB mouse only TL RCI Setting the relief contrast Leica TL RC / TL RCI Operation Leica TL RCI Methods in Transmitted Light Vertical bright-field illuminationSingle-sided dark field Suitable for fixed specimens and fine struc- tures Leica TL RCI Relief Images Starting positionPositive relief contrast Negative relief contrast Dynamic relief contrast Leica TL RCI Relief Images contdSuitable for semitransparent and transparent specimens Filters for Leica TL RC and TL RCI Using FiltersDaylight filter for Leica TL ST Leica IsoPro Non-motorized Controls Operating the Leica IsoPro mechanical stage IsoPro Leica IsoPro Motorized ControlsStage DCI module System Illumination Use Contact with the base If the optics carriers are accidentallyBottom end of the guide rods Back into the original position Using the keyboard Ρρ Use the key to switch the illumination on or offLeica LED5000 MCI contd Illumination scenarios Constraint Leica EL6000 About the Instrument Special featuresHeat-absorbing filter Compatible light guides Leica EL6000 About the Instrument contdIdentification of the instrument Intensity switch Leica EL 6000 OperationControls Running-time meter Leica EL 6000 Troubleshooting Fault description Possible faults RemedyOpen shutter or rotate intensity switch Type only see Cleaning and Maintenance Spare parts and accessoriesReplacing the instrument fuse Repairs Fluorescence Simple filter holders also protect the system from dust Filter ChangerFilter sets for fluorescence FIM Fluorescence Intensity Manager About Fluorescence Microscopy Functional principleLeica M Series Filter system Rapid Filter Changers and Filter Types Simple filter holders Simple filter holdersLonger than 15 seconds Without fluorescence Equipping the Filter Changer Removing the simple filter holderInserting the filter Slot for filter slide Tion Observation without FluorescenceObservation without Fluorescence Double Iris Aperture Burner, you must adjust the arc lamp imme Commissioning the Fluorescence SystemFor observation with fluorescence light Manual M2-216-1 Accessories Leica SmartMove Leica SmartTouch Upper part of the knob = focus Leica PSC Controller ConnectionPassing over the specimen quickly Fine control of the mechanical stage Dimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC / Leica M205 FA Leica M165 FC Leica M205 FA Optical data Leica M165 FC / Leica M205 FA cont’d Encoded/automated M205 FAFIM, filter Engageable zoom notches For repetitive tasks Column, with power supply Computer interface Additional objectives non-parfocalTransmitted light stands With Rottermann Contrast and Ccic Misc. accessories Leica M Stereomicroscopes The Modular System, M1-105-4en Leica EL6000 Specifications Leica EL6000 Specifications contd Illumination types Leica TL ST Transmitted-light BaseInternal shutter/lamp control Integrated filter holder Yes Internal shutter/lamp control Yes Integrated filter holder Leica TL Bfdf Transmitted-light BaseConcave mirror *** With external light source Active f=10mm, end tube f=13mm Weight Leica TL RC / TL RCILeica TL RC Leica TL RCI Concave mirror ****With external light source Compatibility Leica IsoPro Motorized XY StageControl options Appendix Example ParameterCalculation example Magnification in the binocular tube Care, Maintenance, Contact Persons Warranty benefitsContact address Care Mail contact Care, Maintenance, Contact Persons contd Cleaning polymer componentsProtection from dirt Permitted measures