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Leica M205 FA user manual Leica M Series for Fluorescence

Models: M205 FA

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The Leica M Series for Fluorescence

Leica M-series	User Manual	12

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Leica M205 FA user manual Leica M Series for Fluorescence

Contents

Leica M165 FC Leica M205 FA General Instructions Important Safety Notes Special manuals are provided for a number of accessoriesSpecial manuals Symbols Used Important informationExplanatory notes Safety Instructions DescriptionIntended use Non-intended useSafety Instructions contd Radiation from reaching the eyes Beam paths to protect the eyesUV radiation could damage the eyes. Therefore Lamp housing Supply unitMercury lamp Contents Iris Diaphragm Objective NosepieceDifferent Types of Objectives Care, Maintenance, Contact Persons 151 137150 Leica M Series for Fluorescence Congratulations Step Towards Infinity Limits are made to be brokenReliability for your experiments Fewer cablesModular Design Everything is Relative Have a special request? Let us knowMaximum Compatibility ObjectivesTubes EyepiecesOn We Go Assembly Assembling the Focusing Column for MDG Bases Tools usedΡρ Hex socket screwdriver, 3 mm Assembling the Focusing Column with an Incident-light Base AssemblyReadjusting the motorized focus Motorized Focus Restricting the Travel PathRestricting the bottom travel range Tighten the screw of the limit stopOptics Carrier Ρρ Hex socket screwdriver, 4 mmTube Ρρ No tools requiredPreparations Assembling the tubeEyepieces Tools used PreparationMagnification range Objective Attaching the objectiveAlternative fastening options UV Protection Screen Assembly Safety NotesUse with one objective Objective Nosepiece Assembly Do not fall onto the stage plateRemove the transport anchor from the objective nosepiece Screw the optics carrier onto the objective nosepieceObjective Nosepiece Adjusting Parfocality AdjustmentTighten the locking screws Unpacking the base Focusing drive and column Below using the four hexagon-headTransmitted-light Base TL ST Base is delivered with the adapter plateFluoCombi III Preparations Screw the 5× planapochromat into the FluoCombiFluoCombi III Assembly FluoCombi III Assembly contd Assembling the optics carrierFluoCombi III Adjusting the Parfocality Setting up the objective nosepieceBe carried out once. It ensures that Under the beam pathFluoCombi III Adjusting the Parfocality contd FluoCombi III Centering the Objectives Objective is changedEasier Centering the objectiveFluoCombi III Filter and UV Protection Screen Inserting the dichroic mirrorPull the dummy filter carrier out of the holder Assembling the UV protection screenRemoving the transport anchors Lutely necessary to remove the two transport anchorsStage assembly Transmitted-light Base TL BfdfStandard delivery Standard stageTL RC / TL RCI IsoPro Manual Mechanical Stage Assembly Leica IsoPro Mechanical StageLeft or right operation Leica IsoPro Manual Mechanical Stage Assembly contd Control assemblyMechanical stage assembly Place the mechanical stage onto the baseInsert the glass plate into the mechanical stage Leica IsoPro Motorized Mechanical Stage Assembly BasicsLeica IsoPro Motorized Mechanical Stage Assembly contd Future transportBases Mechanical stage and baseMechanical stage for X-Y stage control module Cables Connections Ρρ The interface to the optics carrier is on the columnTerminals Cables Cable Duct Feeding the cablesCables Diagram Alternative installationIng stirrups on both top holes on the drive housing Leica LED5000 MCIHand and tightly screw the retain ConstraintsLeica LED5000 MCI Alternative Assembly Installation on the columnPull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 RL Leica EL6000 Safety Notes General safety notesRights to any warranty claims Such cases, the Declaration of Conformity shall be invalidLeica EL6000 Safety Notes contd Use the instrument in rooms with explosion hazardElectrical safety Setting up the instrument Instructions for lamp replacementLeica EL6000 Preparations Inserting the lampLeica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Leica EL6000 Replacing the Lamp Replacing the lampLeica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Adjusting the interpupillary distance Correct Interpupillary DistanceReference value Look into the eyepiecesUsing the Eyepieces Dioptric correctionIf you do not wear glasses If you wear glassesFocusing FocusingCoarse/fine adjustment Adjusting the Resistance of the Focus Drive Adjusting the resistanceFocusing Changing Magnification Zoom ZoomingLook into the eyepieces Focus on the specimen Ratchet Steps and Magnification Levels Enabling and disabling ratchet stepsMagnifications and fields of view Parfocality More Comfort and Convenience for Your Work Requirements for parfocal workParfocality Enlarge the view to the maximum level Focus on the specimenIris Diaphragm Closing the iris diaphragmOpening the Iris Diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M seriesHealth Notes Potential sources of infectionUsing the Dioptric Correction Dioptric CorrectionAdjusting Dioptric Correction and ParfocalityGraticules UseUse with the Leica AX carrier Convergence angle in the stereoscopic imagePhotography & Video Photography & Video AdapterLeica DFC cameras Leica Application SuitePhoto Tubes and C-mounts Trinocular Video/Phototube 50% Photo beam pathSwitchover Trinocular Video/Phototube 100%Microscope Carrier Objective Nosepiece Parfocal workObjective change Technical constraintsObjectives Optical Accessories Different Types of Objectives Achromatic objectivesPlanachromatic objective 1× Achromatic objectives with a long focal lengthBases Leica TL ST Transmitted-light Base Controls Leica TL ST Transmitted-light Base Operation Light intensity controlTransmitted-light control Bright fieldPrecautionary measures Leica TL ST Transmitted-light Base Changing BulbsChanging the halogen lamp Adapter at the focusing drive Leica TL Bfdf Transmitted-light Base ControlsLeica TL Bfdf Transmitted-light Base Operation Manufacturers of the light guide and cold light sourceDark field Leica TL RC / TL RCI Controls Leica TL RCI The Deflection Mirror Inverted world?Tilted mirror Functions of the rotary knobLeica TL RCI Color Intensity and Temperature Using a USB mouse only TL RCISetting the relief contrast Leica TL RC / TL RCI OperationLeica TL RCI Methods in Transmitted Light Vertical bright-field illuminationSingle-sided dark field Suitable for fixed specimens and fine struc- turesLeica TL RCI Relief Images Starting positionPositive relief contrast Negative relief contrastLeica TL RCI Relief Images contd Dynamic relief contrastSuitable for semitransparent and transparent specimens Using Filters Filters for Leica TL RC and TL RCIDaylight filter for Leica TL ST Leica IsoPro Non-motorized Controls Operating the Leica IsoPro mechanical stageLeica IsoPro Motorized Controls IsoProStage DCI module System Illumination Use Contact with the base If the optics carriers are accidentallyBottom end of the guide rods Back into the original positionUsing the keyboard Ρρ Use the key to switch the illumination on or offLeica LED5000 MCI contd Illumination scenariosConstraint Special features Leica EL6000 About the InstrumentHeat-absorbing filter Leica EL6000 About the Instrument contd Compatible light guidesIdentification of the instrument Intensity switch Leica EL 6000 OperationControls Running-time meterLeica EL 6000 Troubleshooting Fault description Possible faults RemedyOpen shutter or rotate intensity switch Type only seeCleaning and Maintenance Spare parts and accessoriesReplacing the instrument fuse RepairsFluorescence Filter Changer Simple filter holders also protect the system from dustFilter sets for fluorescence FIM Fluorescence Intensity Manager About Fluorescence Microscopy Functional principleLeica M Series Filter systemRapid Filter Changers and Filter Types Simple filter holders Simple filter holdersLonger than 15 seconds Without fluorescenceEquipping the Filter Changer Removing the simple filter holderInserting the filter Slot for filter slideObservation without Fluorescence TionObservation without Fluorescence Double Iris Aperture Burner, you must adjust the arc lamp imme Commissioning the Fluorescence SystemFor observation with fluorescence light Manual M2-216-1Accessories Leica SmartMove Leica SmartTouch Upper part of the knob = focusLeica PSC Controller ConnectionPassing over the specimen quickly Fine control of the mechanical stageDimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC / Leica M205 FA Leica M165 FC Leica M205 FA Optical dataLeica M165 FC / Leica M205 FA cont’d Encoded/automated M205 FAFIM, filter Engageable zoom notches For repetitive tasksColumn, with power supply Computer interface Additional objectives non-parfocalTransmitted light stands With Rottermann Contrast and CcicMisc. accessories Leica M Stereomicroscopes The Modular System, M1-105-4enLeica EL6000 Specifications Leica EL6000 Specifications contd Leica TL ST Transmitted-light Base Illumination typesInternal shutter/lamp control Integrated filter holder Yes Leica TL Bfdf Transmitted-light Base Internal shutter/lamp control Yes Integrated filter holderConcave mirror *** With external light source Active f=10mm, end tube f=13mm Weight Leica TL RC / TL RCILeica TL RC Leica TL RCI Concave mirror ****With external light sourceLeica IsoPro Motorized XY Stage CompatibilityControl options Appendix Parameter ExampleCalculation example Magnification in the binocular tube Care, Maintenance, Contact Persons Warranty benefitsContact address Care Mail contactCare, Maintenance, Contact Persons contd Cleaning polymer componentsProtection from dirt Permitted measures