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Leica M205 FA user manual Overview of an M Series Microscope

Models: M205 FA

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Page 65

Overview of an M Series Microscope

1Eyepieces for spectacle wearers with dioptric correction and eyecups

2Trinocular tube

3Fluorescence housing

4Filter Changer

5Display

6Motorized Focus

7Objective Nosepiece

8Leica SmartTouch

9UV protection screen

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Leica M-series	User Manual	65

Image 65

Leica M205 FA user manual Overview of an M Series Microscope

Contents

Leica M165 FC Leica M205 FA General Instructions Special manuals Important Safety NotesSpecial manuals are provided for a number of accessories Explanatory notes Symbols UsedImportant information Description Safety InstructionsIntended use Non-intended useSafety Instructions contd UV radiation could damage the eyes. Therefore Radiation from reaching the eyesBeam paths to protect the eyes Mercury lamp Lamp housingSupply unit Contents Different Types of Objectives Iris DiaphragmObjective Nosepiece 150 Care, Maintenance, Contact Persons 151137 Leica M Series for Fluorescence Congratulations Limits are made to be broken Step Towards InfinityFewer cables Reliability for your experimentsHave a special request? Let us know Modular Design Everything is RelativeObjectives Maximum CompatibilityTubes EyepiecesOn We Go Assembly Ρρ Hex socket screwdriver, 3 mm Assembling the Focusing Column for MDG BasesTools used Assembly Assembling the Focusing Column with an Incident-light BaseMotorized Focus Restricting the Travel Path Readjusting the motorized focusRestricting the bottom travel range Tighten the screw of the limit stopΡρ Hex socket screwdriver, 4 mm Optics CarrierΡρ No tools required TubePreparations Assembling the tubeMagnification range EyepiecesTools used Preparation Alternative fastening options ObjectiveAttaching the objective Use with one objective UV Protection Screen AssemblySafety Notes Do not fall onto the stage plate Objective Nosepiece AssemblyRemove the transport anchor from the objective nosepiece Screw the optics carrier onto the objective nosepieceTighten the locking screws Objective Nosepiece Adjusting ParfocalityAdjustment Below using the four hexagon-head Unpacking the base Focusing drive and columnTransmitted-light Base TL ST Base is delivered with the adapter plateScrew the 5× planapochromat into the FluoCombi FluoCombi III PreparationsFluoCombi III Assembly Assembling the optics carrier FluoCombi III Assembly contdSetting up the objective nosepiece FluoCombi III Adjusting the ParfocalityBe carried out once. It ensures that Under the beam pathFluoCombi III Adjusting the Parfocality contd Objective is changed FluoCombi III Centering the ObjectivesEasier Centering the objectiveInserting the dichroic mirror FluoCombi III Filter and UV Protection ScreenPull the dummy filter carrier out of the holder Assembling the UV protection screenLutely necessary to remove the two transport anchors Removing the transport anchorsTransmitted-light Base TL Bfdf Stage assemblyStandard delivery Standard stageTL RC / TL RCI Left or right operation IsoPro Manual Mechanical Stage AssemblyLeica IsoPro Mechanical Stage Control assembly Leica IsoPro Manual Mechanical Stage Assembly contdMechanical stage assembly Place the mechanical stage onto the baseInsert the glass plate into the mechanical stage Basics Leica IsoPro Motorized Mechanical Stage AssemblyFuture transport Leica IsoPro Motorized Mechanical Stage Assembly contdMechanical stage and base BasesMechanical stage for X-Y stage control module Terminals Cables ConnectionsΡρ The interface to the optics carrier is on the column Feeding the cables Cables Cable DuctAlternative installation Cables DiagramLeica LED5000 MCI Ing stirrups on both top holes on the drive housingHand and tightly screw the retain ConstraintsPull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 MCI Alternative AssemblyInstallation on the column Leica LED5000 RL General safety notes Leica EL6000 Safety NotesRights to any warranty claims Such cases, the Declaration of Conformity shall be invalidUse the instrument in rooms with explosion hazard Leica EL6000 Safety Notes contdElectrical safety Instructions for lamp replacement Setting up the instrumentLeica EL6000 Preparations Inserting the lampLeica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Replacing the lamp Leica EL6000 Replacing the LampLeica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Correct Interpupillary Distance Adjusting the interpupillary distanceReference value Look into the eyepiecesDioptric correction Using the EyepiecesIf you do not wear glasses If you wear glassesCoarse/fine adjustment FocusingFocusing Focusing Adjusting the Resistance of the Focus DriveAdjusting the resistance Look into the eyepieces Focus on the specimen Changing Magnification ZoomZooming Magnifications and fields of view Ratchet Steps and Magnification LevelsEnabling and disabling ratchet steps Requirements for parfocal work Parfocality More Comfort and Convenience for Your WorkParfocality Enlarge the view to the maximum level Focus on the specimenOpening the Iris Diaphragm Iris DiaphragmClosing the iris diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the EyepiecesPotential sources of infection Health NotesDioptric Correction Using the Dioptric CorrectionDioptric Correction and Parfocality AdjustingUse GraticulesUse with the Leica AX carrier Convergence angle in the stereoscopic imagePhotography & Video Adapter Photography & VideoLeica DFC cameras Leica Application SuitePhoto Tubes and C-mounts Photo beam path Trinocular Video/Phototube 50%Trinocular Video/Phototube 100% SwitchoverMicroscope Carrier Parfocal work Objective NosepieceObjective change Technical constraintsObjectives Optical Accessories Achromatic objectives Different Types of ObjectivesPlanachromatic objective 1× Achromatic objectives with a long focal lengthBases Leica TL ST Transmitted-light Base Controls Light intensity control Leica TL ST Transmitted-light Base OperationTransmitted-light control Bright fieldChanging the halogen lamp Precautionary measuresLeica TL ST Transmitted-light Base Changing Bulbs Leica TL Bfdf Transmitted-light Base Controls Adapter at the focusing driveDark field Leica TL Bfdf Transmitted-light Base OperationManufacturers of the light guide and cold light source Leica TL RC / TL RCI Controls Inverted world? Leica TL RCI The Deflection MirrorTilted mirror Functions of the rotary knobUsing a USB mouse only TL RCI Leica TL RCI Color Intensity and TemperatureLeica TL RC / TL RCI Operation Setting the relief contrastVertical bright-field illumination Leica TL RCI Methods in Transmitted LightSingle-sided dark field Suitable for fixed specimens and fine struc- turesStarting position Leica TL RCI Relief ImagesPositive relief contrast Negative relief contrastSuitable for semitransparent and transparent specimens Leica TL RCI Relief Images contdDynamic relief contrast Daylight filter for Leica TL ST Using FiltersFilters for Leica TL RC and TL RCI Operating the Leica IsoPro mechanical stage Leica IsoPro Non-motorized ControlsStage DCI module Leica IsoPro Motorized ControlsIsoPro System Illumination If the optics carriers are accidentally Use Contact with the baseBottom end of the guide rods Back into the original positionΡρ Use the key to switch the illumination on or off Using the keyboardLeica LED5000 MCI contd Illumination scenariosConstraint Heat-absorbing filter Special featuresLeica EL6000 About the Instrument Identification of the instrument Leica EL6000 About the Instrument contdCompatible light guides Leica EL 6000 Operation Intensity switchControls Running-time meterFault description Possible faults Remedy Leica EL 6000 TroubleshootingOpen shutter or rotate intensity switch Type only seeSpare parts and accessories Cleaning and MaintenanceReplacing the instrument fuse RepairsFluorescence Filter sets for fluorescence Filter ChangerSimple filter holders also protect the system from dust FIM Fluorescence Intensity Manager Functional principle About Fluorescence MicroscopyLeica M Series Filter systemRapid Filter Changers and Filter Types Simple filter holders Simple filter holdersLonger than 15 seconds Without fluorescenceRemoving the simple filter holder Equipping the Filter ChangerInserting the filter Slot for filter slideObservation without Fluorescence Observation without FluorescenceTion Double Iris Aperture Commissioning the Fluorescence System Burner, you must adjust the arc lamp immeFor observation with fluorescence light Manual M2-216-1Accessories Leica SmartMove Upper part of the knob = focus Leica SmartTouchConnection Leica PSC ControllerPassing over the specimen quickly Fine control of the mechanical stageDimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC Leica M205 FA Optical data Leica M165 FC / Leica M205 FAEncoded/automated M205 FA Leica M165 FC / Leica M205 FA cont’dFIM, filter Engageable zoom notches For repetitive tasksAdditional objectives non-parfocal Column, with power supply Computer interfaceTransmitted light stands With Rottermann Contrast and CcicLeica M Stereomicroscopes The Modular System, M1-105-4en Misc. accessoriesLeica EL6000 Specifications Leica EL6000 Specifications contd Internal shutter/lamp control Integrated filter holder Yes Leica TL ST Transmitted-light BaseIllumination types Concave mirror *** With external light source Leica TL Bfdf Transmitted-light BaseInternal shutter/lamp control Yes Integrated filter holder Leica TL RC / TL RCI Active f=10mm, end tube f=13mm WeightLeica TL RC Leica TL RCI Concave mirror ****With external light sourceControl options Leica IsoPro Motorized XY StageCompatibility Appendix Calculation example Magnification in the binocular tube ParameterExample Warranty benefits Care, Maintenance, Contact PersonsContact address Care Mail contactCleaning polymer components Care, Maintenance, Contact Persons contdProtection from dirt Permitted measures