Leica TL RCI Methods in Transmitted Light | Leica M205 FA guide

Leica TL RCI™: Methods in Transmitted Light

Vertical bright-field illumination

Suitable for stained amplitude specimens with sufficient contrast.

The light beams are deflected vertically through the specimen. This results in an accurate bright field with maximum brightness.

Inclined transmitted light

Suitable for semitransparent, opaque speci- mens such as foraminifera and fish eggs. Move the deflection mirror until the desired data are visible.

Single-sided dark field

Suitable for fixed specimens and fine struc- tures.

The flatter the angle at which the light beams are deflected into the specimen plane, the darker the substrate appears. A dark field-like transmitted light is created. Outlines, fine edges and structures are bright, in contrast with the dark background, through diffraction of the light beams on the dark background.

Leica M-series	User Manual	99

Image 99

Leica M205 FA Leica TL RCI Methods in Transmitted Light, Vertical bright-field illumination, Single-sided dark field

Contents

Leica M165 FC Leica M205 FA General Instructions Important Safety Notes Special manuals are provided for a number of accessoriesSpecial manuals Symbols Used Important informationExplanatory notes Non-intended use Safety InstructionsDescription Intended use Safety Instructions contd Radiation from reaching the eyes Beam paths to protect the eyesUV radiation could damage the eyes. Therefore Lamp housing Supply unitMercury lamp Contents Iris Diaphragm Objective NosepieceDifferent Types of Objectives Care, Maintenance, Contact Persons 151 137150 Leica M Series for Fluorescence Congratulations Limits are made to be broken Step Towards Infinity Fewer cables Reliability for your experiments Have a special request? Let us know Modular Design Everything is Relative Eyepieces Maximum CompatibilityObjectives Tubes On We Go Assembly Assembling the Focusing Column for MDG Bases Tools usedΡρ Hex socket screwdriver, 3 mm Assembly Assembling the Focusing Column with an Incident-light Base Tighten the screw of the limit stop Readjusting the motorized focusMotorized Focus Restricting the Travel Path Restricting the bottom travel range Ρρ Hex socket screwdriver, 4 mm Optics Carrier Assembling the tube TubeΡρ No tools required Preparations Eyepieces Tools used PreparationMagnification range Objective Attaching the objectiveAlternative fastening options UV Protection Screen Assembly Safety NotesUse with one objective Screw the optics carrier onto the objective nosepiece Objective Nosepiece AssemblyDo not fall onto the stage plate Remove the transport anchor from the objective nosepiece Objective Nosepiece Adjusting Parfocality AdjustmentTighten the locking screws Base is delivered with the adapter plate Unpacking the base Focusing drive and columnBelow using the four hexagon-head Transmitted-light Base TL ST Screw the 5× planapochromat into the FluoCombi FluoCombi III Preparations FluoCombi III Assembly Assembling the optics carrier FluoCombi III Assembly contd Under the beam path FluoCombi III Adjusting the ParfocalitySetting up the objective nosepiece Be carried out once. It ensures that FluoCombi III Adjusting the Parfocality contd Centering the objective FluoCombi III Centering the ObjectivesObjective is changed Easier Assembling the UV protection screen FluoCombi III Filter and UV Protection ScreenInserting the dichroic mirror Pull the dummy filter carrier out of the holder Lutely necessary to remove the two transport anchors Removing the transport anchors Standard stage Stage assemblyTransmitted-light Base TL Bfdf Standard delivery TL RC / TL RCI IsoPro Manual Mechanical Stage Assembly Leica IsoPro Mechanical StageLeft or right operation Place the mechanical stage onto the base Leica IsoPro Manual Mechanical Stage Assembly contdControl assembly Mechanical stage assembly Insert the glass plate into the mechanical stage Basics Leica IsoPro Motorized Mechanical Stage Assembly Future transport Leica IsoPro Motorized Mechanical Stage Assembly contd Mechanical stage and base Bases Mechanical stage for X-Y stage control module Cables Connections Ρρ The interface to the optics carrier is on the columnTerminals Feeding the cables Cables Cable Duct Alternative installation Cables Diagram Constraints Ing stirrups on both top holes on the drive housingLeica LED5000 MCI Hand and tightly screw the retain Leica LED5000 MCI Alternative Assembly Installation on the columnPull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 RL Such cases, the Declaration of Conformity shall be invalid Leica EL6000 Safety NotesGeneral safety notes Rights to any warranty claims Use the instrument in rooms with explosion hazard Leica EL6000 Safety Notes contd Electrical safety Inserting the lamp Setting up the instrumentInstructions for lamp replacement Leica EL6000 Preparations Leica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Replacing the lamp Leica EL6000 Replacing the Lamp Leica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Look into the eyepieces Adjusting the interpupillary distanceCorrect Interpupillary Distance Reference value If you wear glasses Using the EyepiecesDioptric correction If you do not wear glasses Focusing FocusingCoarse/fine adjustment Adjusting the Resistance of the Focus Drive Adjusting the resistanceFocusing Changing Magnification Zoom ZoomingLook into the eyepieces Focus on the specimen Ratchet Steps and Magnification Levels Enabling and disabling ratchet stepsMagnifications and fields of view Enlarge the view to the maximum level Focus on the specimen Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work Parfocality Iris Diaphragm Closing the iris diaphragmOpening the Iris Diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the Eyepieces Potential sources of infection Health Notes Dioptric Correction Using the Dioptric Correction Dioptric Correction and Parfocality Adjusting Convergence angle in the stereoscopic image GraticulesUse Use with the Leica AX carrier Photography & Video Leica Application Suite Photography & VideoAdapter Leica DFC cameras Photo Tubes and C-mounts Photo beam path Trinocular Video/Phototube 50% Trinocular Video/Phototube 100% Switchover Microscope Carrier Technical constraints Objective NosepieceParfocal work Objective change Objectives Optical Accessories Achromatic objectives with a long focal length Different Types of ObjectivesAchromatic objectives Planachromatic objective 1× Bases Leica TL ST Transmitted-light Base Controls Bright field Leica TL ST Transmitted-light Base OperationLight intensity control Transmitted-light control Precautionary measures Leica TL ST Transmitted-light Base Changing BulbsChanging the halogen lamp Leica TL Bfdf Transmitted-light Base Controls Adapter at the focusing drive Leica TL Bfdf Transmitted-light Base Operation Manufacturers of the light guide and cold light sourceDark field Leica TL RC / TL RCI Controls Functions of the rotary knob Leica TL RCI The Deflection MirrorInverted world? Tilted mirror Using a USB mouse only TL RCI Leica TL RCI Color Intensity and Temperature Leica TL RC / TL RCI Operation Setting the relief contrast Suitable for fixed specimens and fine struc- tures Leica TL RCI Methods in Transmitted LightVertical bright-field illumination Single-sided dark field Negative relief contrast Leica TL RCI Relief ImagesStarting position Positive relief contrast Leica TL RCI Relief Images contd Dynamic relief contrastSuitable for semitransparent and transparent specimens Using Filters Filters for Leica TL RC and TL RCIDaylight filter for Leica TL ST Operating the Leica IsoPro mechanical stage Leica IsoPro Non-motorized Controls Leica IsoPro Motorized Controls IsoProStage DCI module System Illumination Back into the original position Use Contact with the baseIf the optics carriers are accidentally Bottom end of the guide rods Illumination scenarios Using the keyboardΡρ Use the key to switch the illumination on or off Leica LED5000 MCI contd Constraint Special features Leica EL6000 About the InstrumentHeat-absorbing filter Leica EL6000 About the Instrument contd Compatible light guidesIdentification of the instrument Running-time meter Intensity switchLeica EL 6000 Operation Controls Type only see Leica EL 6000 TroubleshootingFault description Possible faults Remedy Open shutter or rotate intensity switch Repairs Cleaning and MaintenanceSpare parts and accessories Replacing the instrument fuse Fluorescence Filter Changer Simple filter holders also protect the system from dustFilter sets for fluorescence FIM Fluorescence Intensity Manager Filter system About Fluorescence MicroscopyFunctional principle Leica M Series Rapid Filter Changers and Filter Types Without fluorescence Simple filter holdersSimple filter holders Longer than 15 seconds Slot for filter slide Equipping the Filter ChangerRemoving the simple filter holder Inserting the filter Observation without Fluorescence TionObservation without Fluorescence Double Iris Aperture Manual M2-216-1 Burner, you must adjust the arc lamp immeCommissioning the Fluorescence System For observation with fluorescence light Accessories Leica SmartMove Upper part of the knob = focus Leica SmartTouch Fine control of the mechanical stage Leica PSC ControllerConnection Passing over the specimen quickly Dimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC Leica M205 FA Optical data Leica M165 FC / Leica M205 FA Engageable zoom notches For repetitive tasks Leica M165 FC / Leica M205 FA cont’dEncoded/automated M205 FA FIM, filter With Rottermann Contrast and Ccic Column, with power supply Computer interfaceAdditional objectives non-parfocal Transmitted light stands Leica M Stereomicroscopes The Modular System, M1-105-4en Misc. accessories Leica EL6000 Specifications Leica EL6000 Specifications contd Leica TL ST Transmitted-light Base Illumination typesInternal shutter/lamp control Integrated filter holder Yes Leica TL Bfdf Transmitted-light Base Internal shutter/lamp control Yes Integrated filter holderConcave mirror *** With external light source Concave mirror ****With external light source Active f=10mm, end tube f=13mm WeightLeica TL RC / TL RCI Leica TL RC Leica TL RCI Leica IsoPro Motorized XY Stage CompatibilityControl options Appendix Parameter ExampleCalculation example Magnification in the binocular tube Mail contact Care, Maintenance, Contact PersonsWarranty benefits Contact address Care Permitted measures Care, Maintenance, Contact Persons contdCleaning polymer components Protection from dirt