Leica M205 FA user manual Observation without Fluorescence, Tion

Observation without Fluorescence

We recommend that you first familiarize yourself with the controls of your Leica fluorescence microscope in transmitted light without the presence of fluorescence illumina-

tion.

•After switching on the high-pressure mercury burner, you must adjust the arc

lamp immediately (see User Manual M2-216-1).

We recommend that you first familiarize yourself with the basic functions of your Leica stereomicroscope as they are described in

the previous section of this User Manual. These include the binocular tube, the eyepieces, the zoom magnification changer, the focusing drive, and others.

The following describes the specific functions of the fluorescence system only.

Observation without Fluorescence

The FLUOIII® filter system includes a UV shutter that can be used to close the illumination beam path. This feature protects the mercury lamp, which should not be switched on and off too frequently. If, for instance, you need to inter- rupt your work, you do not have to switch off the high-pressure mercury burner to preserve sensitive specimens from being burned out or bleached out.

Switching the high-pressure mercury burner on and off frequently decreases its life and causes delays, as the lamp can only

be switched back on after it has cooled. Instead, you can close the UV shutter and reopen it when you return to your workplace.

Close the UV shutter when you do not want to view the specimen with fluorescence illumina- tion, but only with transmitted light.

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