Leica TL RCI Relief Images contd | Leica M205 FA instruction

Leica TL RCI™: Relief Images (cont'd.)

Dynamic relief contrast

Suitable for semitransparent and transparent specimens.

By tilting the deflection mirror slightly, you can move the gaps over the entire field of view and quickly toggle between positive and negative relief images. The dynamic effect makes it easy to distinguish phase structures from amplitude structures.

Constraints

The relief methods provide good results from mid-zoom to high magnifications and with 1×, 1.6× and 2× objectives. In the lower zoom half and with weaker specimens, the object field may not be uniformly illuminated.

We recommend using the transmitted-light base with 1× or higher objectives, and not objectives with a long focal length.

Leica M-series	User Manual	101

Image 101

Leica M205 FA user manual Leica TL RCI Relief Images contd, Dynamic relief contrast

Contents

Leica M165 FC Leica M205 FA General Instructions Special manuals Important Safety NotesSpecial manuals are provided for a number of accessories Explanatory notes Symbols UsedImportant information Description Safety InstructionsIntended use Non-intended use Safety Instructions contd UV radiation could damage the eyes. Therefore Radiation from reaching the eyesBeam paths to protect the eyes Mercury lamp Lamp housingSupply unit Contents Different Types of Objectives Iris DiaphragmObjective Nosepiece 150 Care, Maintenance, Contact Persons 151137 Leica M Series for Fluorescence Congratulations Limits are made to be broken Step Towards Infinity Fewer cables Reliability for your experiments Have a special request? Let us know Modular Design Everything is Relative Objectives Maximum CompatibilityTubes Eyepieces On We Go Assembly Ρρ Hex socket screwdriver, 3 mm Assembling the Focusing Column for MDG BasesTools used Assembly Assembling the Focusing Column with an Incident-light Base Motorized Focus Restricting the Travel Path Readjusting the motorized focusRestricting the bottom travel range Tighten the screw of the limit stop Ρρ Hex socket screwdriver, 4 mm Optics Carrier Ρρ No tools required TubePreparations Assembling the tube Magnification range EyepiecesTools used Preparation Alternative fastening options ObjectiveAttaching the objective Use with one objective UV Protection Screen AssemblySafety Notes Do not fall onto the stage plate Objective Nosepiece AssemblyRemove the transport anchor from the objective nosepiece Screw the optics carrier onto the objective nosepiece Tighten the locking screws Objective Nosepiece Adjusting ParfocalityAdjustment Below using the four hexagon-head Unpacking the base Focusing drive and columnTransmitted-light Base TL ST Base is delivered with the adapter plate Screw the 5× planapochromat into the FluoCombi FluoCombi III Preparations FluoCombi III Assembly Assembling the optics carrier FluoCombi III Assembly contd Setting up the objective nosepiece FluoCombi III Adjusting the ParfocalityBe carried out once. It ensures that Under the beam path FluoCombi III Adjusting the Parfocality contd Objective is changed FluoCombi III Centering the ObjectivesEasier Centering the objective Inserting the dichroic mirror FluoCombi III Filter and UV Protection ScreenPull the dummy filter carrier out of the holder Assembling the UV protection screen Lutely necessary to remove the two transport anchors Removing the transport anchors Transmitted-light Base TL Bfdf Stage assemblyStandard delivery Standard stage TL RC / TL RCI Left or right operation IsoPro Manual Mechanical Stage AssemblyLeica IsoPro Mechanical Stage Control assembly Leica IsoPro Manual Mechanical Stage Assembly contdMechanical stage assembly Place the mechanical stage onto the base Insert the glass plate into the mechanical stage Basics Leica IsoPro Motorized Mechanical Stage Assembly Future transport Leica IsoPro Motorized Mechanical Stage Assembly contd Mechanical stage and base Bases Mechanical stage for X-Y stage control module Terminals Cables ConnectionsΡρ The interface to the optics carrier is on the column Feeding the cables Cables Cable Duct Alternative installation Cables Diagram Leica LED5000 MCI Ing stirrups on both top holes on the drive housingHand and tightly screw the retain Constraints Pull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 MCI Alternative AssemblyInstallation on the column Leica LED5000 RL General safety notes Leica EL6000 Safety NotesRights to any warranty claims Such cases, the Declaration of Conformity shall be invalid Use the instrument in rooms with explosion hazard Leica EL6000 Safety Notes contd Electrical safety Instructions for lamp replacement Setting up the instrumentLeica EL6000 Preparations Inserting the lamp Leica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Replacing the lamp Leica EL6000 Replacing the Lamp Leica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Correct Interpupillary Distance Adjusting the interpupillary distanceReference value Look into the eyepieces Dioptric correction Using the EyepiecesIf you do not wear glasses If you wear glasses Coarse/fine adjustment FocusingFocusing Focusing Adjusting the Resistance of the Focus DriveAdjusting the resistance Look into the eyepieces Focus on the specimen Changing Magnification ZoomZooming Magnifications and fields of view Ratchet Steps and Magnification LevelsEnabling and disabling ratchet steps Requirements for parfocal work Parfocality More Comfort and Convenience for Your WorkParfocality Enlarge the view to the maximum level Focus on the specimen Opening the Iris Diaphragm Iris DiaphragmClosing the iris diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the Eyepieces Potential sources of infection Health Notes Dioptric Correction Using the Dioptric Correction Dioptric Correction and Parfocality Adjusting Use GraticulesUse with the Leica AX carrier Convergence angle in the stereoscopic image Photography & Video Adapter Photography & VideoLeica DFC cameras Leica Application Suite Photo Tubes and C-mounts Photo beam path Trinocular Video/Phototube 50% Trinocular Video/Phototube 100% Switchover Microscope Carrier Parfocal work Objective NosepieceObjective change Technical constraints Objectives Optical Accessories Achromatic objectives Different Types of ObjectivesPlanachromatic objective 1× Achromatic objectives with a long focal length Bases Leica TL ST Transmitted-light Base Controls Light intensity control Leica TL ST Transmitted-light Base OperationTransmitted-light control Bright field Changing the halogen lamp Precautionary measuresLeica TL ST Transmitted-light Base Changing Bulbs Leica TL Bfdf Transmitted-light Base Controls Adapter at the focusing drive Dark field Leica TL Bfdf Transmitted-light Base OperationManufacturers of the light guide and cold light source Leica TL RC / TL RCI Controls Inverted world? Leica TL RCI The Deflection MirrorTilted mirror Functions of the rotary knob Using a USB mouse only TL RCI Leica TL RCI Color Intensity and Temperature Leica TL RC / TL RCI Operation Setting the relief contrast Vertical bright-field illumination Leica TL RCI Methods in Transmitted LightSingle-sided dark field Suitable for fixed specimens and fine struc- tures Starting position Leica TL RCI Relief ImagesPositive relief contrast Negative relief contrast Suitable for semitransparent and transparent specimens Leica TL RCI Relief Images contdDynamic relief contrast Daylight filter for Leica TL ST Using FiltersFilters for Leica TL RC and TL RCI Operating the Leica IsoPro mechanical stage Leica IsoPro Non-motorized Controls Stage DCI module Leica IsoPro Motorized ControlsIsoPro System Illumination If the optics carriers are accidentally Use Contact with the baseBottom end of the guide rods Back into the original position Ρρ Use the key to switch the illumination on or off Using the keyboardLeica LED5000 MCI contd Illumination scenarios Constraint Heat-absorbing filter Special featuresLeica EL6000 About the Instrument Identification of the instrument Leica EL6000 About the Instrument contdCompatible light guides Leica EL 6000 Operation Intensity switchControls Running-time meter Fault description Possible faults Remedy Leica EL 6000 TroubleshootingOpen shutter or rotate intensity switch Type only see Spare parts and accessories Cleaning and MaintenanceReplacing the instrument fuse Repairs Fluorescence Filter sets for fluorescence Filter ChangerSimple filter holders also protect the system from dust FIM Fluorescence Intensity Manager Functional principle About Fluorescence MicroscopyLeica M Series Filter system Rapid Filter Changers and Filter Types Simple filter holders Simple filter holdersLonger than 15 seconds Without fluorescence Removing the simple filter holder Equipping the Filter ChangerInserting the filter Slot for filter slide Observation without Fluorescence Observation without FluorescenceTion Double Iris Aperture Commissioning the Fluorescence System Burner, you must adjust the arc lamp immeFor observation with fluorescence light Manual M2-216-1 Accessories Leica SmartMove Upper part of the knob = focus Leica SmartTouch Connection Leica PSC ControllerPassing over the specimen quickly Fine control of the mechanical stage Dimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC Leica M205 FA Optical data Leica M165 FC / Leica M205 FA Encoded/automated M205 FA Leica M165 FC / Leica M205 FA cont’dFIM, filter Engageable zoom notches For repetitive tasks Additional objectives non-parfocal Column, with power supply Computer interfaceTransmitted light stands With Rottermann Contrast and Ccic Leica M Stereomicroscopes The Modular System, M1-105-4en Misc. accessories Leica EL6000 Specifications Leica EL6000 Specifications contd Internal shutter/lamp control Integrated filter holder Yes Leica TL ST Transmitted-light BaseIllumination types Concave mirror *** With external light source Leica TL Bfdf Transmitted-light BaseInternal shutter/lamp control Yes Integrated filter holder Leica TL RC / TL RCI Active f=10mm, end tube f=13mm WeightLeica TL RC Leica TL RCI Concave mirror ****With external light source Control options Leica IsoPro Motorized XY StageCompatibility Appendix Calculation example Magnification in the binocular tube ParameterExample Warranty benefits Care, Maintenance, Contact PersonsContact address Care Mail contact Cleaning polymer components Care, Maintenance, Contact Persons contdProtection from dirt Permitted measures