Leica M205 FA Microscope & Magnifier Manual | Fluorescence Stereomicroscopy

About Fluorescence Microscopy

Functional principle

Fluorescent substances light up when irradi- ated with short-wave excitation light, and fluo- rescence microscopy takes advantage of this property. Specimens without self-fluorescence are stained with a fluorescent substance. One such dye is green fluorescent protein (GFP), which is used in molecular biology.

The Leica M Series

The Leica M series fluorescence stereomicro- scopes allow entire fluorescent specimens to be studied, unprepared and in three dimen- sions. The fully apochromatic optics system and the enormous zoom range make the Leica M series stereomicroscopes your first choice. The patented TripleBeam® third beam path for fluo- rescence illumination and the patented FLUOIII® filter system generate highest-quality fluores- cence images. The very bright high-pressure mercury burner and specially selected filter sets enable you to distinguish the finest structures and enhance the quantity of information for incident light fluorescence.

The filter system

The FLUOIII® filter system consists of a rapid filter changer for barrier and excitation filters and a filter insert for a filter holder with your choice of filter. A total of four filter sets (which are labeled) can be used in the rapid filter changer. An empty filter cartridge is also avail- able for individual filter combinations. Each filter set includes two barrier filters to the visual beam paths and an excitation filter to the illu- mination beam path.

Leica M-series	User Manual	117

Image 117

Leica M205 FA user manual About Fluorescence Microscopy, Functional principle, Leica M Series, Filter system

Contents

Leica M165 FC Leica M205 FA General Instructions Important Safety Notes Special manuals are provided for a number of accessoriesSpecial manuals Symbols Used Important informationExplanatory notes Description Safety InstructionsIntended use Non-intended use Safety Instructions contd Radiation from reaching the eyes Beam paths to protect the eyesUV radiation could damage the eyes. Therefore Lamp housing Supply unitMercury lamp Contents Iris Diaphragm Objective NosepieceDifferent Types of Objectives Care, Maintenance, Contact Persons 151 137150 Leica M Series for Fluorescence Congratulations Limits are made to be broken Step Towards Infinity Fewer cables Reliability for your experiments Have a special request? Let us know Modular Design Everything is Relative Objectives Maximum CompatibilityTubes Eyepieces On We Go Assembly Assembling the Focusing Column for MDG Bases Tools usedΡρ Hex socket screwdriver, 3 mm Assembly Assembling the Focusing Column with an Incident-light Base Motorized Focus Restricting the Travel Path Readjusting the motorized focusRestricting the bottom travel range Tighten the screw of the limit stop Ρρ Hex socket screwdriver, 4 mm Optics Carrier Ρρ No tools required TubePreparations Assembling the tube Eyepieces Tools used PreparationMagnification range Objective Attaching the objectiveAlternative fastening options UV Protection Screen Assembly Safety NotesUse with one objective Do not fall onto the stage plate Objective Nosepiece AssemblyRemove the transport anchor from the objective nosepiece Screw the optics carrier onto the objective nosepiece Objective Nosepiece Adjusting Parfocality AdjustmentTighten the locking screws Below using the four hexagon-head Unpacking the base Focusing drive and columnTransmitted-light Base TL ST Base is delivered with the adapter plate Screw the 5× planapochromat into the FluoCombi FluoCombi III Preparations FluoCombi III Assembly Assembling the optics carrier FluoCombi III Assembly contd Setting up the objective nosepiece FluoCombi III Adjusting the ParfocalityBe carried out once. It ensures that Under the beam path FluoCombi III Adjusting the Parfocality contd Objective is changed FluoCombi III Centering the ObjectivesEasier Centering the objective Inserting the dichroic mirror FluoCombi III Filter and UV Protection ScreenPull the dummy filter carrier out of the holder Assembling the UV protection screen Lutely necessary to remove the two transport anchors Removing the transport anchors Transmitted-light Base TL Bfdf Stage assemblyStandard delivery Standard stage TL RC / TL RCI IsoPro Manual Mechanical Stage Assembly Leica IsoPro Mechanical StageLeft or right operation Control assembly Leica IsoPro Manual Mechanical Stage Assembly contdMechanical stage assembly Place the mechanical stage onto the base Insert the glass plate into the mechanical stage Basics Leica IsoPro Motorized Mechanical Stage Assembly Future transport Leica IsoPro Motorized Mechanical Stage Assembly contd Mechanical stage and base Bases Mechanical stage for X-Y stage control module Cables Connections Ρρ The interface to the optics carrier is on the columnTerminals Feeding the cables Cables Cable Duct Alternative installation Cables Diagram Leica LED5000 MCI Ing stirrups on both top holes on the drive housingHand and tightly screw the retain Constraints Leica LED5000 MCI Alternative Assembly Installation on the columnPull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 RL General safety notes Leica EL6000 Safety NotesRights to any warranty claims Such cases, the Declaration of Conformity shall be invalid Use the instrument in rooms with explosion hazard Leica EL6000 Safety Notes contd Electrical safety Instructions for lamp replacement Setting up the instrumentLeica EL6000 Preparations Inserting the lamp Leica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Replacing the lamp Leica EL6000 Replacing the Lamp Leica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Correct Interpupillary Distance Adjusting the interpupillary distanceReference value Look into the eyepieces Dioptric correction Using the EyepiecesIf you do not wear glasses If you wear glasses Focusing FocusingCoarse/fine adjustment Adjusting the Resistance of the Focus Drive Adjusting the resistanceFocusing Changing Magnification Zoom ZoomingLook into the eyepieces Focus on the specimen Ratchet Steps and Magnification Levels Enabling and disabling ratchet stepsMagnifications and fields of view Requirements for parfocal work Parfocality More Comfort and Convenience for Your WorkParfocality Enlarge the view to the maximum level Focus on the specimen Iris Diaphragm Closing the iris diaphragmOpening the Iris Diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the Eyepieces Potential sources of infection Health Notes Dioptric Correction Using the Dioptric Correction Dioptric Correction and Parfocality Adjusting Use GraticulesUse with the Leica AX carrier Convergence angle in the stereoscopic image Photography & Video Adapter Photography & VideoLeica DFC cameras Leica Application Suite Photo Tubes and C-mounts Photo beam path Trinocular Video/Phototube 50% Trinocular Video/Phototube 100% Switchover Microscope Carrier Parfocal work Objective NosepieceObjective change Technical constraints Objectives Optical Accessories Achromatic objectives Different Types of ObjectivesPlanachromatic objective 1× Achromatic objectives with a long focal length Bases Leica TL ST Transmitted-light Base Controls Light intensity control Leica TL ST Transmitted-light Base OperationTransmitted-light control Bright field Precautionary measures Leica TL ST Transmitted-light Base Changing BulbsChanging the halogen lamp Leica TL Bfdf Transmitted-light Base Controls Adapter at the focusing drive Leica TL Bfdf Transmitted-light Base Operation Manufacturers of the light guide and cold light sourceDark field Leica TL RC / TL RCI Controls Inverted world? Leica TL RCI The Deflection MirrorTilted mirror Functions of the rotary knob Using a USB mouse only TL RCI Leica TL RCI Color Intensity and Temperature Leica TL RC / TL RCI Operation Setting the relief contrast Vertical bright-field illumination Leica TL RCI Methods in Transmitted LightSingle-sided dark field Suitable for fixed specimens and fine struc- tures Starting position Leica TL RCI Relief ImagesPositive relief contrast Negative relief contrast Leica TL RCI Relief Images contd Dynamic relief contrastSuitable for semitransparent and transparent specimens Using Filters Filters for Leica TL RC and TL RCIDaylight filter for Leica TL ST Operating the Leica IsoPro mechanical stage Leica IsoPro Non-motorized Controls Leica IsoPro Motorized Controls IsoProStage DCI module System Illumination If the optics carriers are accidentally Use Contact with the baseBottom end of the guide rods Back into the original position Ρρ Use the key to switch the illumination on or off Using the keyboardLeica LED5000 MCI contd Illumination scenarios Constraint Special features Leica EL6000 About the InstrumentHeat-absorbing filter Leica EL6000 About the Instrument contd Compatible light guidesIdentification of the instrument Leica EL 6000 Operation Intensity switchControls Running-time meter Fault description Possible faults Remedy Leica EL 6000 TroubleshootingOpen shutter or rotate intensity switch Type only see Spare parts and accessories Cleaning and MaintenanceReplacing the instrument fuse Repairs Fluorescence Filter Changer Simple filter holders also protect the system from dustFilter sets for fluorescence FIM Fluorescence Intensity Manager Functional principle About Fluorescence MicroscopyLeica M Series Filter system Rapid Filter Changers and Filter Types Simple filter holders Simple filter holdersLonger than 15 seconds Without fluorescence Removing the simple filter holder Equipping the Filter ChangerInserting the filter Slot for filter slide Observation without Fluorescence TionObservation without Fluorescence Double Iris Aperture Commissioning the Fluorescence System Burner, you must adjust the arc lamp immeFor observation with fluorescence light Manual M2-216-1 Accessories Leica SmartMove Upper part of the knob = focus Leica SmartTouch Connection Leica PSC ControllerPassing over the specimen quickly Fine control of the mechanical stage Dimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC Leica M205 FA Optical data Leica M165 FC / Leica M205 FA Encoded/automated M205 FA Leica M165 FC / Leica M205 FA cont’dFIM, filter Engageable zoom notches For repetitive tasks Additional objectives non-parfocal Column, with power supply Computer interfaceTransmitted light stands With Rottermann Contrast and Ccic Leica M Stereomicroscopes The Modular System, M1-105-4en Misc. accessories Leica EL6000 Specifications Leica EL6000 Specifications contd Leica TL ST Transmitted-light Base Illumination typesInternal shutter/lamp control Integrated filter holder Yes Leica TL Bfdf Transmitted-light Base Internal shutter/lamp control Yes Integrated filter holderConcave mirror *** With external light source Leica TL RC / TL RCI Active f=10mm, end tube f=13mm WeightLeica TL RC Leica TL RCI Concave mirror ****With external light source Leica IsoPro Motorized XY Stage CompatibilityControl options Appendix Parameter ExampleCalculation example Magnification in the binocular tube Warranty benefits Care, Maintenance, Contact PersonsContact address Care Mail contact Cleaning polymer components Care, Maintenance, Contact Persons contdProtection from dirt Permitted measures