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Leica M205 FA user manual Iris Diaphragm, Objective Nosepiece, Different Types of Objectives

Models: M205 FA

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Quick Start Guide
The Fastest Route to Success	64
Overview of an M Series Microscope	65
The Correct Interpupillary Distance	66
Using the Eyepieces	67
Focusing	68
Adjusting the Resistance of the Focus Drive	69
Changing Magnification (Zoom)	70
Ratchet Steps and Magnification Levels	71
Parfocality: More Comfort and Convenience for Your Work	72
Iris Diaphragm	73

Eyepieces
Magnification Factors of the Eyepieces	75
Health Notes	76
Dioptric Correction	77
Dioptric Correction and Parfocality	78
Graticules	79

Photography & Video
Photography & Video	81
Photo Tubes and C-mounts	82
Trinocular Video/Phototube 50%	83
Trinocular Video/Phototube 100%	84

Microscope Carrier
The Objective Nosepiece	86

Objectives andOptical Accessories
The Different Types of Objectives	88

Bases
Leica TL ST Transmitted-light Base: Controls	90
Leica TL ST Transmitted-light Base: Operation	91
Leica TL ST Transmitted-light Base: Changing Bulbs	92
Leica TL BFDF Transmitted-light Base: Controls	93
Leica TL BFDF Transmitted-light Base: Operation	94
Leica TL RC™ / TL RCI™: Controls	95
Leica TL RCI™: The Deflection Mirror	96
Leica TL RCI™: Color Intensity and Temperature	97
Leica TL RC™ / TL RCI™: Operation	98
Leica TL RCI™: Methods in Transmitted Light	99
Leica TL RCI™: Relief Images	100
Using Filters	102
Leica IsoPro™ (Non-motorized): Controls	103
Leica IsoPro™ (Motorized): Controls	104

Leica M-series	User Manual	10

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Leica M205 FA user manual Iris Diaphragm, Objective Nosepiece, Different Types of Objectives

Contents

Leica M165 FC Leica M205 FA General Instructions Special manuals are provided for a number of accessories Important Safety NotesSpecial manuals Important information Symbols UsedExplanatory notes Intended use Safety InstructionsDescription Non-intended useSafety Instructions contd Beam paths to protect the eyes Radiation from reaching the eyesUV radiation could damage the eyes. Therefore Supply unit Lamp housingMercury lamp Contents Objective Nosepiece Iris DiaphragmDifferent Types of Objectives 137 Care, Maintenance, Contact Persons 151150 Leica M Series for Fluorescence Congratulations Step Towards Infinity Limits are made to be brokenReliability for your experiments Fewer cablesModular Design Everything is Relative Have a special request? Let us knowTubes Maximum CompatibilityObjectives EyepiecesOn We Go Assembly Tools used Assembling the Focusing Column for MDG BasesΡρ Hex socket screwdriver, 3 mm Assembling the Focusing Column with an Incident-light Base AssemblyRestricting the bottom travel range Readjusting the motorized focusMotorized Focus Restricting the Travel Path Tighten the screw of the limit stopOptics Carrier Ρρ Hex socket screwdriver, 4 mmPreparations TubeΡρ No tools required Assembling the tubeTools used Preparation EyepiecesMagnification range Attaching the objective ObjectiveAlternative fastening options Safety Notes UV Protection Screen AssemblyUse with one objective Remove the transport anchor from the objective nosepiece Objective Nosepiece AssemblyDo not fall onto the stage plate Screw the optics carrier onto the objective nosepieceAdjustment Objective Nosepiece Adjusting ParfocalityTighten the locking screws Transmitted-light Base TL ST Unpacking the base Focusing drive and columnBelow using the four hexagon-head Base is delivered with the adapter plateFluoCombi III Preparations Screw the 5× planapochromat into the FluoCombiFluoCombi III Assembly FluoCombi III Assembly contd Assembling the optics carrierBe carried out once. It ensures that FluoCombi III Adjusting the ParfocalitySetting up the objective nosepiece Under the beam pathFluoCombi III Adjusting the Parfocality contd Easier FluoCombi III Centering the ObjectivesObjective is changed Centering the objectivePull the dummy filter carrier out of the holder FluoCombi III Filter and UV Protection ScreenInserting the dichroic mirror Assembling the UV protection screenRemoving the transport anchors Lutely necessary to remove the two transport anchorsStandard delivery Stage assemblyTransmitted-light Base TL Bfdf Standard stageTL RC / TL RCI Leica IsoPro Mechanical Stage IsoPro Manual Mechanical Stage AssemblyLeft or right operation Mechanical stage assembly Leica IsoPro Manual Mechanical Stage Assembly contdControl assembly Place the mechanical stage onto the baseInsert the glass plate into the mechanical stage Leica IsoPro Motorized Mechanical Stage Assembly BasicsLeica IsoPro Motorized Mechanical Stage Assembly contd Future transportBases Mechanical stage and baseMechanical stage for X-Y stage control module Ρρ The interface to the optics carrier is on the column Cables ConnectionsTerminals Cables Cable Duct Feeding the cablesCables Diagram Alternative installationHand and tightly screw the retain Ing stirrups on both top holes on the drive housingLeica LED5000 MCI ConstraintsInstallation on the column Leica LED5000 MCI Alternative AssemblyPull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 RL Rights to any warranty claims Leica EL6000 Safety NotesGeneral safety notes Such cases, the Declaration of Conformity shall be invalidLeica EL6000 Safety Notes contd Use the instrument in rooms with explosion hazardElectrical safety Leica EL6000 Preparations Setting up the instrumentInstructions for lamp replacement Inserting the lampLeica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Leica EL6000 Replacing the Lamp Replacing the lampLeica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Reference value Adjusting the interpupillary distanceCorrect Interpupillary Distance Look into the eyepiecesIf you do not wear glasses Using the EyepiecesDioptric correction If you wear glassesFocusing FocusingCoarse/fine adjustment Adjusting the resistance Adjusting the Resistance of the Focus DriveFocusing Zooming Changing Magnification ZoomLook into the eyepieces Focus on the specimen Enabling and disabling ratchet steps Ratchet Steps and Magnification LevelsMagnifications and fields of view Parfocality Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work Enlarge the view to the maximum level Focus on the specimenClosing the iris diaphragm Iris DiaphragmOpening the Iris Diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M seriesHealth Notes Potential sources of infectionUsing the Dioptric Correction Dioptric CorrectionAdjusting Dioptric Correction and ParfocalityUse with the Leica AX carrier GraticulesUse Convergence angle in the stereoscopic imagePhotography & Video Leica DFC cameras Photography & VideoAdapter Leica Application SuitePhoto Tubes and C-mounts Trinocular Video/Phototube 50% Photo beam pathSwitchover Trinocular Video/Phototube 100%Microscope Carrier Objective change Objective NosepieceParfocal work Technical constraintsObjectives Optical Accessories Planachromatic objective 1× Different Types of ObjectivesAchromatic objectives Achromatic objectives with a long focal lengthBases Leica TL ST Transmitted-light Base Controls Transmitted-light control Leica TL ST Transmitted-light Base OperationLight intensity control Bright fieldLeica TL ST Transmitted-light Base Changing Bulbs Precautionary measuresChanging the halogen lamp Adapter at the focusing drive Leica TL Bfdf Transmitted-light Base ControlsManufacturers of the light guide and cold light source Leica TL Bfdf Transmitted-light Base OperationDark field Leica TL RC / TL RCI Controls Tilted mirror Leica TL RCI The Deflection MirrorInverted world? Functions of the rotary knobLeica TL RCI Color Intensity and Temperature Using a USB mouse only TL RCISetting the relief contrast Leica TL RC / TL RCI OperationSingle-sided dark field Leica TL RCI Methods in Transmitted LightVertical bright-field illumination Suitable for fixed specimens and fine struc- turesPositive relief contrast Leica TL RCI Relief ImagesStarting position Negative relief contrastDynamic relief contrast Leica TL RCI Relief Images contdSuitable for semitransparent and transparent specimens Filters for Leica TL RC and TL RCI Using FiltersDaylight filter for Leica TL ST Leica IsoPro Non-motorized Controls Operating the Leica IsoPro mechanical stageIsoPro Leica IsoPro Motorized ControlsStage DCI module System Illumination Bottom end of the guide rods Use Contact with the baseIf the optics carriers are accidentally Back into the original positionLeica LED5000 MCI contd Using the keyboardΡρ Use the key to switch the illumination on or off Illumination scenariosConstraint Leica EL6000 About the Instrument Special featuresHeat-absorbing filter Compatible light guides Leica EL6000 About the Instrument contdIdentification of the instrument Controls Intensity switchLeica EL 6000 Operation Running-time meterOpen shutter or rotate intensity switch Leica EL 6000 TroubleshootingFault description Possible faults Remedy Type only seeReplacing the instrument fuse Cleaning and MaintenanceSpare parts and accessories RepairsFluorescence Simple filter holders also protect the system from dust Filter ChangerFilter sets for fluorescence FIM Fluorescence Intensity Manager Leica M Series About Fluorescence MicroscopyFunctional principle Filter systemRapid Filter Changers and Filter Types Longer than 15 seconds Simple filter holdersSimple filter holders Without fluorescenceInserting the filter Equipping the Filter ChangerRemoving the simple filter holder Slot for filter slideTion Observation without FluorescenceObservation without Fluorescence Double Iris Aperture For observation with fluorescence light Burner, you must adjust the arc lamp immeCommissioning the Fluorescence System Manual M2-216-1Accessories Leica SmartMove Leica SmartTouch Upper part of the knob = focusPassing over the specimen quickly Leica PSC ControllerConnection Fine control of the mechanical stageDimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC / Leica M205 FA Leica M165 FC Leica M205 FA Optical dataFIM, filter Leica M165 FC / Leica M205 FA cont’dEncoded/automated M205 FA Engageable zoom notches For repetitive tasksTransmitted light stands Column, with power supply Computer interfaceAdditional objectives non-parfocal With Rottermann Contrast and CcicMisc. accessories Leica M Stereomicroscopes The Modular System, M1-105-4enLeica EL6000 Specifications Leica EL6000 Specifications contd Illumination types Leica TL ST Transmitted-light BaseInternal shutter/lamp control Integrated filter holder Yes Internal shutter/lamp control Yes Integrated filter holder Leica TL Bfdf Transmitted-light BaseConcave mirror *** With external light source Leica TL RC Leica TL RCI Active f=10mm, end tube f=13mm WeightLeica TL RC / TL RCI Concave mirror ****With external light sourceCompatibility Leica IsoPro Motorized XY StageControl options Appendix Example ParameterCalculation example Magnification in the binocular tube Contact address Care Care, Maintenance, Contact PersonsWarranty benefits Mail contactProtection from dirt Care, Maintenance, Contact Persons contdCleaning polymer components Permitted measures