Leica Adjusting Parfocality for Optimal Focus in Your Microscope

Objective Nosepiece – Adjusting Parfocality

The following procedure only has to be carried out once. Afterwards, both objectives are parfo- cal, meaning that the specimen remains in focus when the objective nosepiece is rotated.

This procedure must be repeated if you replace either of the two objectives with another.

The following example assumes the combi- nation of the 1× and 2× planapochromats. If you are using another objective combination, replace the 2× objective in the description with the objective with the stronger magnification.

Preparation

ρρ	Open the iris diaphragm.
ρρ	Set the dioptric correction of the eyepieces
	to "0".

Adjustment

1.Rotate the 2× objective into the beam path and set it to the lowest magnification.

2.Focus on the specimen.

3.Rotate the 1× objective into the beam path.

4.Turn the objective on the thread in both directions until the specimen appears sharp.

5.Toggle to the 2× objective.

6.Select the strongest magnification and refocus until the specimen appears abso- lutely sharp.

7.Toggle to the 1× objective.

8.Turn the objective on the thread in both directions until the specimen appears absolutely sharp.

By means of zooming, check that the behavior of the objective is parfocal. Repeat the check with the other objective. If it is not parfocal, repeat the procedure.

9. Tighten the locking screws.

Leica M-series User Manual 29