Contents | Leica M205 FA guide

Contents

General Instructions	2
Important Safety Notes	3
Symbols Used	4
Safety Instructions	5

The Leica M Series for Fluorescence
Congratulations!	13
A Step Towards Infinity	14
The Electronics: Comfort, Convenience and Safety	15
The Modular Design: Everything is Relative	16
Maximum Compatibility	17
On We Go	18

Assembly
Assembling the Focusing Column for MDG Bases	20
Assembling the Focusing Column with an Incident-light Base	21
Motorized Focus: Restricting the Travel Path	22
Optics Carrier	23
Tube	24
Eyepieces	25
Objective	26
UV Protection Screen – Assembly	27
Objective Nosepiece – Assembly	28
Objective Nosepiece – Adjusting Parfocality	29

Transmitted-light Base TL ST	30
FluoCombi III™ – Preparations	31
FluoCombi III™ – Assembly	32
FluoCombi III™ – Adjusting the Parfocality	34
FluoCombi III™ – Centering the Objectives	36
FluoCombi III™ – Filter and UV Protection Screen	37
Transmitted-light Base TL BFDF: Before First Use	38
Transmitted-light Base TL BFDF	39
TL RC™ / TL RCI™	40
IsoPro™ Manual Mechanical Stage: Assembly	41
Leica IsoPro™ Motorized Mechanical Stage: Assembly	44
Cables: Connections	48
Cables: Cable Duct	49
Cables: Diagram	50
Leica LED5000 MCI™	51
Leica LED5000 MCI™: Alternative Assembly	52
Leica LED5000 RL	53
Leica EL6000 – Safety Notes	54
Leica EL6000 – Preparations	57
Leica EL6000 – Connection to the Fluorescence Lamp Housing	59
Leica EL6000 – Replacing the Lamp	61

Leica EL6000 – Connection to the Fluorescence Lamp Housing (cont'd.)62

Leica M-series	User Manual	9

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Leica M205 FA user manual Contents

Contents

Leica M165 FC Leica M205 FA General Instructions Important Safety Notes Special manuals are provided for a number of accessoriesSpecial manuals Symbols Used Important informationExplanatory notes Description Safety InstructionsIntended use Non-intended use Safety Instructions contd Radiation from reaching the eyes Beam paths to protect the eyesUV radiation could damage the eyes. Therefore Lamp housing Supply unitMercury lamp Contents Iris Diaphragm Objective NosepieceDifferent Types of Objectives Care, Maintenance, Contact Persons 151 137150 Leica M Series for Fluorescence Congratulations Limits are made to be broken Step Towards Infinity Fewer cables Reliability for your experiments Have a special request? Let us know Modular Design Everything is Relative Objectives Maximum CompatibilityTubes Eyepieces On We Go Assembly Assembling the Focusing Column for MDG Bases Tools usedΡρ Hex socket screwdriver, 3 mm Assembly Assembling the Focusing Column with an Incident-light Base Motorized Focus Restricting the Travel Path Readjusting the motorized focusRestricting the bottom travel range Tighten the screw of the limit stop Ρρ Hex socket screwdriver, 4 mm Optics Carrier Ρρ No tools required TubePreparations Assembling the tube Eyepieces Tools used PreparationMagnification range Objective Attaching the objectiveAlternative fastening options UV Protection Screen Assembly Safety NotesUse with one objective Do not fall onto the stage plate Objective Nosepiece AssemblyRemove the transport anchor from the objective nosepiece Screw the optics carrier onto the objective nosepiece Objective Nosepiece Adjusting Parfocality AdjustmentTighten the locking screws Below using the four hexagon-head Unpacking the base Focusing drive and columnTransmitted-light Base TL ST Base is delivered with the adapter plate Screw the 5× planapochromat into the FluoCombi FluoCombi III Preparations FluoCombi III Assembly Assembling the optics carrier FluoCombi III Assembly contd Setting up the objective nosepiece FluoCombi III Adjusting the ParfocalityBe carried out once. It ensures that Under the beam path FluoCombi III Adjusting the Parfocality contd Objective is changed FluoCombi III Centering the ObjectivesEasier Centering the objective Inserting the dichroic mirror FluoCombi III Filter and UV Protection ScreenPull the dummy filter carrier out of the holder Assembling the UV protection screen Lutely necessary to remove the two transport anchors Removing the transport anchors Transmitted-light Base TL Bfdf Stage assemblyStandard delivery Standard stage TL RC / TL RCI IsoPro Manual Mechanical Stage Assembly Leica IsoPro Mechanical StageLeft or right operation Control assembly Leica IsoPro Manual Mechanical Stage Assembly contdMechanical stage assembly Place the mechanical stage onto the base Insert the glass plate into the mechanical stage Basics Leica IsoPro Motorized Mechanical Stage Assembly Future transport Leica IsoPro Motorized Mechanical Stage Assembly contd Mechanical stage and base Bases Mechanical stage for X-Y stage control module Cables Connections Ρρ The interface to the optics carrier is on the columnTerminals Feeding the cables Cables Cable Duct Alternative installation Cables Diagram Leica LED5000 MCI Ing stirrups on both top holes on the drive housingHand and tightly screw the retain Constraints Leica LED5000 MCI Alternative Assembly Installation on the columnPull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 RL General safety notes Leica EL6000 Safety NotesRights to any warranty claims Such cases, the Declaration of Conformity shall be invalid Use the instrument in rooms with explosion hazard Leica EL6000 Safety Notes contd Electrical safety Instructions for lamp replacement Setting up the instrumentLeica EL6000 Preparations Inserting the lamp Leica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Replacing the lamp Leica EL6000 Replacing the Lamp Leica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Correct Interpupillary Distance Adjusting the interpupillary distanceReference value Look into the eyepieces Dioptric correction Using the EyepiecesIf you do not wear glasses If you wear glasses Focusing FocusingCoarse/fine adjustment Adjusting the Resistance of the Focus Drive Adjusting the resistanceFocusing Changing Magnification Zoom ZoomingLook into the eyepieces Focus on the specimen Ratchet Steps and Magnification Levels Enabling and disabling ratchet stepsMagnifications and fields of view Requirements for parfocal work Parfocality More Comfort and Convenience for Your WorkParfocality Enlarge the view to the maximum level Focus on the specimen Iris Diaphragm Closing the iris diaphragmOpening the Iris Diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the Eyepieces Potential sources of infection Health Notes Dioptric Correction Using the Dioptric Correction Dioptric Correction and Parfocality Adjusting Use GraticulesUse with the Leica AX carrier Convergence angle in the stereoscopic image Photography & Video Adapter Photography & VideoLeica DFC cameras Leica Application Suite Photo Tubes and C-mounts Photo beam path Trinocular Video/Phototube 50% Trinocular Video/Phototube 100% Switchover Microscope Carrier Parfocal work Objective NosepieceObjective change Technical constraints Objectives Optical Accessories Achromatic objectives Different Types of ObjectivesPlanachromatic objective 1× Achromatic objectives with a long focal length Bases Leica TL ST Transmitted-light Base Controls Light intensity control Leica TL ST Transmitted-light Base OperationTransmitted-light control Bright field Precautionary measures Leica TL ST Transmitted-light Base Changing BulbsChanging the halogen lamp Leica TL Bfdf Transmitted-light Base Controls Adapter at the focusing drive Leica TL Bfdf Transmitted-light Base Operation Manufacturers of the light guide and cold light sourceDark field Leica TL RC / TL RCI Controls Inverted world? Leica TL RCI The Deflection MirrorTilted mirror Functions of the rotary knob Using a USB mouse only TL RCI Leica TL RCI Color Intensity and Temperature Leica TL RC / TL RCI Operation Setting the relief contrast Vertical bright-field illumination Leica TL RCI Methods in Transmitted LightSingle-sided dark field Suitable for fixed specimens and fine struc- tures Starting position Leica TL RCI Relief ImagesPositive relief contrast Negative relief contrast Leica TL RCI Relief Images contd Dynamic relief contrastSuitable for semitransparent and transparent specimens Using Filters Filters for Leica TL RC and TL RCIDaylight filter for Leica TL ST Operating the Leica IsoPro mechanical stage Leica IsoPro Non-motorized Controls Leica IsoPro Motorized Controls IsoProStage DCI module System Illumination If the optics carriers are accidentally Use Contact with the baseBottom end of the guide rods Back into the original position Ρρ Use the key to switch the illumination on or off Using the keyboardLeica LED5000 MCI contd Illumination scenarios Constraint Special features Leica EL6000 About the InstrumentHeat-absorbing filter Leica EL6000 About the Instrument contd Compatible light guidesIdentification of the instrument Leica EL 6000 Operation Intensity switchControls Running-time meter Fault description Possible faults Remedy Leica EL 6000 TroubleshootingOpen shutter or rotate intensity switch Type only see Spare parts and accessories Cleaning and MaintenanceReplacing the instrument fuse Repairs Fluorescence Filter Changer Simple filter holders also protect the system from dustFilter sets for fluorescence FIM Fluorescence Intensity Manager Functional principle About Fluorescence MicroscopyLeica M Series Filter system Rapid Filter Changers and Filter Types Simple filter holders Simple filter holdersLonger than 15 seconds Without fluorescence Removing the simple filter holder Equipping the Filter ChangerInserting the filter Slot for filter slide Observation without Fluorescence TionObservation without Fluorescence Double Iris Aperture Commissioning the Fluorescence System Burner, you must adjust the arc lamp immeFor observation with fluorescence light Manual M2-216-1 Accessories Leica SmartMove Upper part of the knob = focus Leica SmartTouch Connection Leica PSC ControllerPassing over the specimen quickly Fine control of the mechanical stage Dimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC Leica M205 FA Optical data Leica M165 FC / Leica M205 FA Encoded/automated M205 FA Leica M165 FC / Leica M205 FA cont’dFIM, filter Engageable zoom notches For repetitive tasks Additional objectives non-parfocal Column, with power supply Computer interfaceTransmitted light stands With Rottermann Contrast and Ccic Leica M Stereomicroscopes The Modular System, M1-105-4en Misc. accessories Leica EL6000 Specifications Leica EL6000 Specifications contd Leica TL ST Transmitted-light Base Illumination typesInternal shutter/lamp control Integrated filter holder Yes Leica TL Bfdf Transmitted-light Base Internal shutter/lamp control Yes Integrated filter holderConcave mirror *** With external light source Leica TL RC / TL RCI Active f=10mm, end tube f=13mm WeightLeica TL RC Leica TL RCI Concave mirror ****With external light source Leica IsoPro Motorized XY Stage CompatibilityControl options Appendix Parameter ExampleCalculation example Magnification in the binocular tube Warranty benefits Care, Maintenance, Contact PersonsContact address Care Mail contact Cleaning polymer components Care, Maintenance, Contact Persons contdProtection from dirt Permitted measures