FIM Fluorescence Intensity Manager | Leica M205 FA instruction

FIM – Fluorescence Intensity Manager

FIM consists of a diaphragm disk with pinhole diaphragms of varying transmission capaci- ties. The intensity of the excitation light can be reduced in five fixed steps. The light can be changed from 100% to 55%, 30% and 17% down to 10%.

The advantage of this procedure is its absolute reproducibility and fast switching between the intensity levels. A separate FIM level can be stored for every fluorescence filter. Different intensities of fluorescence can thus be cali- brated to one another.

Leica M-series	User Manual	116

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Leica M205 FA user manual FIM Fluorescence Intensity Manager

Contents

Leica M165 FC Leica M205 FA General Instructions Special manuals Important Safety NotesSpecial manuals are provided for a number of accessories Explanatory notes Symbols UsedImportant information Safety Instructions DescriptionIntended use Non-intended use Safety Instructions contd UV radiation could damage the eyes. Therefore Radiation from reaching the eyesBeam paths to protect the eyes Mercury lamp Lamp housingSupply unit Contents Different Types of Objectives Iris DiaphragmObjective Nosepiece 150 Care, Maintenance, Contact Persons 151137 Leica M Series for Fluorescence Congratulations Step Towards Infinity Limits are made to be broken Reliability for your experiments Fewer cables Modular Design Everything is Relative Have a special request? Let us know Maximum Compatibility ObjectivesTubes Eyepieces On We Go Assembly Ρρ Hex socket screwdriver, 3 mm Assembling the Focusing Column for MDG BasesTools used Assembling the Focusing Column with an Incident-light Base Assembly Readjusting the motorized focus Motorized Focus Restricting the Travel PathRestricting the bottom travel range Tighten the screw of the limit stop Optics Carrier Ρρ Hex socket screwdriver, 4 mm Tube Ρρ No tools requiredPreparations Assembling the tube Magnification range EyepiecesTools used Preparation Alternative fastening options ObjectiveAttaching the objective Use with one objective UV Protection Screen AssemblySafety Notes Objective Nosepiece Assembly Do not fall onto the stage plateRemove the transport anchor from the objective nosepiece Screw the optics carrier onto the objective nosepiece Tighten the locking screws Objective Nosepiece Adjusting ParfocalityAdjustment Unpacking the base Focusing drive and column Below using the four hexagon-headTransmitted-light Base TL ST Base is delivered with the adapter plate FluoCombi III Preparations Screw the 5× planapochromat into the FluoCombi FluoCombi III Assembly FluoCombi III Assembly contd Assembling the optics carrier FluoCombi III Adjusting the Parfocality Setting up the objective nosepieceBe carried out once. It ensures that Under the beam path FluoCombi III Adjusting the Parfocality contd FluoCombi III Centering the Objectives Objective is changedEasier Centering the objective FluoCombi III Filter and UV Protection Screen Inserting the dichroic mirrorPull the dummy filter carrier out of the holder Assembling the UV protection screen Removing the transport anchors Lutely necessary to remove the two transport anchors Stage assembly Transmitted-light Base TL BfdfStandard delivery Standard stage TL RC / TL RCI Left or right operation IsoPro Manual Mechanical Stage AssemblyLeica IsoPro Mechanical Stage Leica IsoPro Manual Mechanical Stage Assembly contd Control assemblyMechanical stage assembly Place the mechanical stage onto the base Insert the glass plate into the mechanical stage Leica IsoPro Motorized Mechanical Stage Assembly Basics Leica IsoPro Motorized Mechanical Stage Assembly contd Future transport Bases Mechanical stage and base Mechanical stage for X-Y stage control module Terminals Cables ConnectionsΡρ The interface to the optics carrier is on the column Cables Cable Duct Feeding the cables Cables Diagram Alternative installation Ing stirrups on both top holes on the drive housing Leica LED5000 MCIHand and tightly screw the retain Constraints Pull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 MCI Alternative AssemblyInstallation on the column Leica LED5000 RL Leica EL6000 Safety Notes General safety notesRights to any warranty claims Such cases, the Declaration of Conformity shall be invalid Leica EL6000 Safety Notes contd Use the instrument in rooms with explosion hazard Electrical safety Setting up the instrument Instructions for lamp replacementLeica EL6000 Preparations Inserting the lamp Leica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Leica EL6000 Replacing the Lamp Replacing the lamp Leica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Adjusting the interpupillary distance Correct Interpupillary DistanceReference value Look into the eyepieces Using the Eyepieces Dioptric correctionIf you do not wear glasses If you wear glasses Coarse/fine adjustment FocusingFocusing Focusing Adjusting the Resistance of the Focus DriveAdjusting the resistance Look into the eyepieces Focus on the specimen Changing Magnification ZoomZooming Magnifications and fields of view Ratchet Steps and Magnification LevelsEnabling and disabling ratchet steps Parfocality More Comfort and Convenience for Your Work Requirements for parfocal workParfocality Enlarge the view to the maximum level Focus on the specimen Opening the Iris Diaphragm Iris DiaphragmClosing the iris diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M series Health Notes Potential sources of infection Using the Dioptric Correction Dioptric Correction Adjusting Dioptric Correction and Parfocality Graticules UseUse with the Leica AX carrier Convergence angle in the stereoscopic image Photography & Video Photography & Video AdapterLeica DFC cameras Leica Application Suite Photo Tubes and C-mounts Trinocular Video/Phototube 50% Photo beam path Switchover Trinocular Video/Phototube 100% Microscope Carrier Objective Nosepiece Parfocal workObjective change Technical constraints Objectives Optical Accessories Different Types of Objectives Achromatic objectivesPlanachromatic objective 1× Achromatic objectives with a long focal length Bases Leica TL ST Transmitted-light Base Controls Leica TL ST Transmitted-light Base Operation Light intensity controlTransmitted-light control Bright field Changing the halogen lamp Precautionary measuresLeica TL ST Transmitted-light Base Changing Bulbs Adapter at the focusing drive Leica TL Bfdf Transmitted-light Base Controls Dark field Leica TL Bfdf Transmitted-light Base OperationManufacturers of the light guide and cold light source Leica TL RC / TL RCI Controls Leica TL RCI The Deflection Mirror Inverted world?Tilted mirror Functions of the rotary knob Leica TL RCI Color Intensity and Temperature Using a USB mouse only TL RCI Setting the relief contrast Leica TL RC / TL RCI Operation Leica TL RCI Methods in Transmitted Light Vertical bright-field illuminationSingle-sided dark field Suitable for fixed specimens and fine struc- tures Leica TL RCI Relief Images Starting positionPositive relief contrast Negative relief contrast Suitable for semitransparent and transparent specimens Leica TL RCI Relief Images contdDynamic relief contrast Daylight filter for Leica TL ST Using FiltersFilters for Leica TL RC and TL RCI Leica IsoPro Non-motorized Controls Operating the Leica IsoPro mechanical stage Stage DCI module Leica IsoPro Motorized ControlsIsoPro System Illumination Use Contact with the base If the optics carriers are accidentallyBottom end of the guide rods Back into the original position Using the keyboard Ρρ Use the key to switch the illumination on or offLeica LED5000 MCI contd Illumination scenarios Constraint Heat-absorbing filter Special featuresLeica EL6000 About the Instrument Identification of the instrument Leica EL6000 About the Instrument contdCompatible light guides Intensity switch Leica EL 6000 OperationControls Running-time meter Leica EL 6000 Troubleshooting Fault description Possible faults RemedyOpen shutter or rotate intensity switch Type only see Cleaning and Maintenance Spare parts and accessoriesReplacing the instrument fuse Repairs Fluorescence Filter sets for fluorescence Filter ChangerSimple filter holders also protect the system from dust FIM Fluorescence Intensity Manager About Fluorescence Microscopy Functional principleLeica M Series Filter system Rapid Filter Changers and Filter Types Simple filter holders Simple filter holdersLonger than 15 seconds Without fluorescence Equipping the Filter Changer Removing the simple filter holderInserting the filter Slot for filter slide Observation without Fluorescence Observation without FluorescenceTion Double Iris Aperture Burner, you must adjust the arc lamp imme Commissioning the Fluorescence SystemFor observation with fluorescence light Manual M2-216-1 Accessories Leica SmartMove Leica SmartTouch Upper part of the knob = focus Leica PSC Controller ConnectionPassing over the specimen quickly Fine control of the mechanical stage Dimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC / Leica M205 FA Leica M165 FC Leica M205 FA Optical data Leica M165 FC / Leica M205 FA cont’d Encoded/automated M205 FAFIM, filter Engageable zoom notches For repetitive tasks Column, with power supply Computer interface Additional objectives non-parfocalTransmitted light stands With Rottermann Contrast and Ccic Misc. accessories Leica M Stereomicroscopes The Modular System, M1-105-4en Leica EL6000 Specifications Leica EL6000 Specifications contd Internal shutter/lamp control Integrated filter holder Yes Leica TL ST Transmitted-light BaseIllumination types Concave mirror *** With external light source Leica TL Bfdf Transmitted-light BaseInternal shutter/lamp control Yes Integrated filter holder Active f=10mm, end tube f=13mm Weight Leica TL RC / TL RCILeica TL RC Leica TL RCI Concave mirror ****With external light source Control options Leica IsoPro Motorized XY StageCompatibility Appendix Calculation example Magnification in the binocular tube ParameterExample Care, Maintenance, Contact Persons Warranty benefitsContact address Care Mail contact Care, Maintenance, Contact Persons contd Cleaning polymer componentsProtection from dirt Permitted measures