Fluorescence | Leica M205 FA guide

Fluorescence

Leica M-series	User Manual	114

Image 114

Leica M205 FA user manual Fluorescence

Contents

Leica M165 FC Leica M205 FA General Instructions Important Safety Notes Special manuals are provided for a number of accessoriesSpecial manuals Symbols Used Important informationExplanatory notes Intended use Safety InstructionsDescription Non-intended use Safety Instructions contd Radiation from reaching the eyes Beam paths to protect the eyesUV radiation could damage the eyes. Therefore Lamp housing Supply unitMercury lamp Contents Iris Diaphragm Objective NosepieceDifferent Types of Objectives Care, Maintenance, Contact Persons 151 137150 Leica M Series for Fluorescence Congratulations Step Towards Infinity Limits are made to be broken Reliability for your experiments Fewer cables Modular Design Everything is Relative Have a special request? Let us know Tubes Maximum CompatibilityObjectives Eyepieces On We Go Assembly Assembling the Focusing Column for MDG Bases Tools usedΡρ Hex socket screwdriver, 3 mm Assembling the Focusing Column with an Incident-light Base Assembly Restricting the bottom travel range Readjusting the motorized focusMotorized Focus Restricting the Travel Path Tighten the screw of the limit stop Optics Carrier Ρρ Hex socket screwdriver, 4 mm Preparations TubeΡρ No tools required Assembling the tube Eyepieces Tools used PreparationMagnification range Objective Attaching the objectiveAlternative fastening options UV Protection Screen Assembly Safety NotesUse with one objective Remove the transport anchor from the objective nosepiece Objective Nosepiece AssemblyDo not fall onto the stage plate Screw the optics carrier onto the objective nosepiece Objective Nosepiece Adjusting Parfocality AdjustmentTighten the locking screws Transmitted-light Base TL ST Unpacking the base Focusing drive and columnBelow using the four hexagon-head Base is delivered with the adapter plate FluoCombi III Preparations Screw the 5× planapochromat into the FluoCombi FluoCombi III Assembly FluoCombi III Assembly contd Assembling the optics carrier Be carried out once. It ensures that FluoCombi III Adjusting the ParfocalitySetting up the objective nosepiece Under the beam path FluoCombi III Adjusting the Parfocality contd Easier FluoCombi III Centering the ObjectivesObjective is changed Centering the objective Pull the dummy filter carrier out of the holder FluoCombi III Filter and UV Protection ScreenInserting the dichroic mirror Assembling the UV protection screen Removing the transport anchors Lutely necessary to remove the two transport anchors Standard delivery Stage assemblyTransmitted-light Base TL Bfdf Standard stage TL RC / TL RCI IsoPro Manual Mechanical Stage Assembly Leica IsoPro Mechanical StageLeft or right operation Mechanical stage assembly Leica IsoPro Manual Mechanical Stage Assembly contdControl assembly Place the mechanical stage onto the base Insert the glass plate into the mechanical stage Leica IsoPro Motorized Mechanical Stage Assembly Basics Leica IsoPro Motorized Mechanical Stage Assembly contd Future transport Bases Mechanical stage and base Mechanical stage for X-Y stage control module Cables Connections Ρρ The interface to the optics carrier is on the columnTerminals Cables Cable Duct Feeding the cables Cables Diagram Alternative installation Hand and tightly screw the retain Ing stirrups on both top holes on the drive housingLeica LED5000 MCI Constraints Leica LED5000 MCI Alternative Assembly Installation on the columnPull the retaining stirrup out of the Leica LED5000 MCI Leica LED5000 RL Rights to any warranty claims Leica EL6000 Safety NotesGeneral safety notes Such cases, the Declaration of Conformity shall be invalid Leica EL6000 Safety Notes contd Use the instrument in rooms with explosion hazard Electrical safety Leica EL6000 Preparations Setting up the instrumentInstructions for lamp replacement Inserting the lamp Leica EL6000 Preparation contd Leica EL6000 Connection to the Fluorescence Lamp Housing Leica EL6000 Preparation contd Leica EL6000 Replacing the Lamp Replacing the lamp Leica M-series Quick Start Guide Fastest Route to Success Overview of an M Series Microscope Reference value Adjusting the interpupillary distanceCorrect Interpupillary Distance Look into the eyepieces If you do not wear glasses Using the EyepiecesDioptric correction If you wear glasses Focusing FocusingCoarse/fine adjustment Adjusting the Resistance of the Focus Drive Adjusting the resistanceFocusing Changing Magnification Zoom ZoomingLook into the eyepieces Focus on the specimen Ratchet Steps and Magnification Levels Enabling and disabling ratchet stepsMagnifications and fields of view Parfocality Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work Enlarge the view to the maximum level Focus on the specimen Iris Diaphragm Closing the iris diaphragmOpening the Iris Diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M series Health Notes Potential sources of infection Using the Dioptric Correction Dioptric Correction Adjusting Dioptric Correction and Parfocality Use with the Leica AX carrier GraticulesUse Convergence angle in the stereoscopic image Photography & Video Leica DFC cameras Photography & VideoAdapter Leica Application Suite Photo Tubes and C-mounts Trinocular Video/Phototube 50% Photo beam path Switchover Trinocular Video/Phototube 100% Microscope Carrier Objective change Objective NosepieceParfocal work Technical constraints Objectives Optical Accessories Planachromatic objective 1× Different Types of ObjectivesAchromatic objectives Achromatic objectives with a long focal length Bases Leica TL ST Transmitted-light Base Controls Transmitted-light control Leica TL ST Transmitted-light Base OperationLight intensity control Bright field Precautionary measures Leica TL ST Transmitted-light Base Changing BulbsChanging the halogen lamp Adapter at the focusing drive Leica TL Bfdf Transmitted-light Base Controls Leica TL Bfdf Transmitted-light Base Operation Manufacturers of the light guide and cold light sourceDark field Leica TL RC / TL RCI Controls Tilted mirror Leica TL RCI The Deflection MirrorInverted world? Functions of the rotary knob Leica TL RCI Color Intensity and Temperature Using a USB mouse only TL RCI Setting the relief contrast Leica TL RC / TL RCI Operation Single-sided dark field Leica TL RCI Methods in Transmitted LightVertical bright-field illumination Suitable for fixed specimens and fine struc- tures Positive relief contrast Leica TL RCI Relief ImagesStarting position Negative relief contrast Leica TL RCI Relief Images contd Dynamic relief contrastSuitable for semitransparent and transparent specimens Using Filters Filters for Leica TL RC and TL RCIDaylight filter for Leica TL ST Leica IsoPro Non-motorized Controls Operating the Leica IsoPro mechanical stage Leica IsoPro Motorized Controls IsoProStage DCI module System Illumination Bottom end of the guide rods Use Contact with the baseIf the optics carriers are accidentally Back into the original position Leica LED5000 MCI contd Using the keyboardΡρ Use the key to switch the illumination on or off Illumination scenarios Constraint Special features Leica EL6000 About the InstrumentHeat-absorbing filter Leica EL6000 About the Instrument contd Compatible light guidesIdentification of the instrument Controls Intensity switchLeica EL 6000 Operation Running-time meter Open shutter or rotate intensity switch Leica EL 6000 TroubleshootingFault description Possible faults Remedy Type only see Replacing the instrument fuse Cleaning and MaintenanceSpare parts and accessories Repairs Fluorescence Filter Changer Simple filter holders also protect the system from dustFilter sets for fluorescence FIM Fluorescence Intensity Manager Leica M Series About Fluorescence MicroscopyFunctional principle Filter system Rapid Filter Changers and Filter Types Longer than 15 seconds Simple filter holdersSimple filter holders Without fluorescence Inserting the filter Equipping the Filter ChangerRemoving the simple filter holder Slot for filter slide Observation without Fluorescence TionObservation without Fluorescence Double Iris Aperture For observation with fluorescence light Burner, you must adjust the arc lamp immeCommissioning the Fluorescence System Manual M2-216-1 Accessories Leica SmartMove Leica SmartTouch Upper part of the knob = focus Passing over the specimen quickly Leica PSC ControllerConnection Fine control of the mechanical stage Dimensional Drawings Leica M165 FC Leica M165 FC contd Leica M-series 131 Leica M205 FA Leica M205 FA contd Leica M-series 134 Leica M-series 135 Leica M-series 136 Mats TL Specifications Leica M165 FC / Leica M205 FA Leica M165 FC Leica M205 FA Optical data FIM, filter Leica M165 FC / Leica M205 FA cont’dEncoded/automated M205 FA Engageable zoom notches For repetitive tasks Transmitted light stands Column, with power supply Computer interfaceAdditional objectives non-parfocal With Rottermann Contrast and Ccic Misc. accessories Leica M Stereomicroscopes The Modular System, M1-105-4en Leica EL6000 Specifications Leica EL6000 Specifications contd Leica TL ST Transmitted-light Base Illumination typesInternal shutter/lamp control Integrated filter holder Yes Leica TL Bfdf Transmitted-light Base Internal shutter/lamp control Yes Integrated filter holderConcave mirror *** With external light source Leica TL RC Leica TL RCI Active f=10mm, end tube f=13mm WeightLeica TL RC / TL RCI Concave mirror ****With external light source Leica IsoPro Motorized XY Stage CompatibilityControl options Appendix Parameter ExampleCalculation example Magnification in the binocular tube Contact address Care Care, Maintenance, Contact PersonsWarranty benefits Mail contact Protection from dirt Care, Maintenance, Contact Persons contdCleaning polymer components Permitted measures